Testing sustained soil-to-soil contact as an approach for limiting the abiotic influence of source soils during experimental microbiome transfer
Experimental separation of the biotic and abiotic components of soil will help in understanding the role of taxonomy and composition in soil microbiome function. The most common approach to soil microbiome transfer involves direct dilution of a non-sterile source soil into sterile recipient soils, introducing both microorganisms and soil compounds, leaving abiotic and biotic factors confounded. Here, we contrast microbiome transfer into sterile recipient soils through (i) direct soil transfer at two dilutions and (ii) a new approach, sustained contact between source and recipient soils. Sustained soil-to-soil contact retains separation between source and recipient soils, allows for multiple colonization events and increases confidence that microorganisms observed in recipient soils are active and growing. Each approach produced distinct microbiomes in recipient soils after 1 and 6 weeks of incubation, indicating that transfer method impacts microbial composition. The extent to which recipient microbiomes resembled source microbiomes varied by soil type, although in general, direct soil transfer appeared to most closely approximate source microbiomes. However, irrespective of transfer method, most bacterial sequences in recipient soils were from organisms transferred through all methods. We discuss the merits of each method for controlled soil microbiome studies.
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Work Title Testing sustained soil-to-soil contact as an approach for limiting the abiotic influence of source soils during experimental microbiome transfer Access Creators - Ryan Vincent Trexler
- Terrence Bell
License CC BY-NC-ND 4.0 (Attribution-NonCommercial-NoDerivatives) Work Type Article Publisher - FEMS Microbiology Letters
Publication Date 2019 Publisher Identifier (DOI) - 10.1093/femsle/fnz228
Related URLs Deposited March 25, 2021 Versions
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Version 1
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Added Creator Ryan Vincent Trexler
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Added Creator Terrence Bell
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MicrobiomeTransfer_PostPrint.pdf -
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Supplemental_MicrobiomeTransfer_FEMS.pdf -
Updated License Show ChangesLicense
- https://creativecommons.org/licenses/by-nc-nd/4.0/
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Published
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Updated Publisher Identifier (DOI), Related URLs Show ChangesPublisher Identifier (DOI)Related URLs
https://doi.org/10.1093/femsle/fnz228- 10.1093/femsle/fnz228
- https://doi.org/10.1093/femsle/fnz228
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