Characterization of cyanobacterial allophycocyanins absorbing far-red light
Phycobiliproteins (PBPs) are pigment proteins that comprise phycobilisomes (PBS), major light-harvesting antenna complexes of cyanobacteria and red algae. PBS core substructures are made up of allophycocyanins (APs), a subfamily of PBPs. Five paralogous AP subunits are encoded by the Far-Red Light Photoacclimation (FaRLiP) gene cluster, which is transcriptionally activated in cells grown in far-red light (FRL; lambda = 700 to 800 nm). FaRLiP gene expression enables some terrestrial cyanobacteria to remodel their PBS and photosystems and perform oxygenic photosynthesis in far-red light (FRL). Paralogous AP genes encoding a putative, FRL-absorbing AP (FRL-AP) are also found in an operon associated with improved low-light growth (LL; <50 μmol photons m–2 s–1) in some thermophilic Synechococcus spp., a phenomenon termed Low-Light Photoacclimation (LoLiP). In this study, apc genes from FaRLiP and LoLiP gene clusters were heterologously expressed individually and in combinations in Escherichia coli. The resulting novel FRL-APs were characterized and identified as major contributors to the FRL absorbance observed in whole-cells after FaRLiP and potentially in LoLiP. Post-translational modifications of native FRL-APs from FaRLiP cyanobacterium, Leptolyngbya sp. strain JSC-1, were analyzed by mass spectrometry. The PBP complexes made in two FaRLiP organisms were compared, revealing strain-specific diversity in the FaRLiP responses of cyanobacteria. Through analyses of native and recombinant proteins, we improved our understanding of how different cyanobacterial strains utilize specialized APs to acclimate to FRL and LL. We discuss some insights into structural changes that may allow these APs to absorb longer light wavelengths than their visible-light-absorbing paralogs.
Files
-
View: PRES D-20-00068-R1 MS.pdf
Download: PRES D-20-00068-R1 MS.pdf
Metadata
Work Title Characterization of cyanobacterial allophycocyanins absorbing far-red light Access Creators - Don Bryant
- Nathan Soulier , Tatiana N. Laremore
Keyword - Photosynthesis; phycobiliproteins; allophycocyanin; phycobilisomes; far-red light photoacclimation; low-light photoacclimation
License In Copyright (Rights Reserved) Work Type Article Publisher - Springer
Publication Date 2020 Subject - Photosynthesis
Language - English
Publisher Identifier (DOI) - doi: 10.1007/s11120-020-00775-2
Source - Photosynthesis Research
Deposited February 23, 2021 Versions
Analytics
Collections
This resource is currently not in any collection.
Work History
Version 1
published-
Created
-
Added Creator Don Bryant
-
Added
PRES D-20-00068-R1 MS.pdf -
Added
PRES D-20-00068-R1 Supplement.pdf -
Updated License Show ChangesLicense
- https://rightsstatements.org/page/InC/1.0/
-
Published
-
Added Creator Nathan Soulier , Tatiana N. Laremore
-
Updated
-
Updated