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Effect of operating pressure on protein fouling during constant-pressure virus removal filtration

Virus removal filtration provides a robust size-based removal of enveloped and non-enveloped viruses in the production of monoclonal antibodies and serum-derived products. The filtrate flux in virus removal filtration is typically dominated by membrane fouling associated with the therapeutic protein product, but very few studies have examined the effect of operating pressure on the fouling rate/mechanisms or the virus filter capacity. Filtration experiments were performed using Viresolve® Pro virus removal filters with human serum Immunoglobulin G (hIgG) as a model protein over a range of operating pressures from 10 to 60 psi. The extent of fouling (evaluated in terms of the overall resistance at a given throughput) decreased with increasing operating pressure up to about 30 psi, while the filter capacity (evaluated using the Vmax model) increased with increasing pressure. The flux decline behavior was shown to be in good agreement with the combined complete pore blockage and cake filtration model, with the pore blockage parameter decreasing with increasing pressure, while the cake filtration parameter increased with increasing pressure. Confocal microscopy was used to identify the location and extent of protein deposition within the membrane cross-section, providing further support for the effects of pressure on both pore blockage and cake resistance. These results provide important insights into the factors controlling protein fouling during virus removal filtration and the influence of operating pressure on the flux decline and filter capacity.

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Metadata

Work Title Effect of operating pressure on protein fouling during constant-pressure virus removal filtration
Access
Open Access
Creators
  1. Joshua Peles
  2. Fatemeh Fallahianbijan
  3. Benjamin Cacace
  4. Christina Carbrello
  5. Sal Giglia
  6. Andrew L. Zydney
Keyword
  1. Virus filtration
  2. Monoclonal antibody
  3. Bioprocessing
  4. Fouling
License CC BY-NC-ND 4.0 (Attribution-NonCommercial-NoDerivatives)
Work Type Article
Publisher
  1. Journal of Membrane Science
Publication Date April 15, 2022
Publisher Identifier (DOI)
  1. https://doi.org/10.1016/j.memsci.2022.120351
Deposited July 21, 2022

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Work History

Version 1
published

  • Created

    July 21, 2022 12:44 by Researcher Metadata Database

  • Added Viresolve_Pro_fouling-pressure_effects-unmarked.docx

    July 21, 2022 12:44 by Researcher Metadata Database

  • Added Creator Joshua Peles

    July 21, 2022 12:44 by Researcher Metadata Database

  • Added Creator Fatemeh Fallahianbijan

    July 21, 2022 12:44 by Researcher Metadata Database

  • Added Creator Benjamin Cacace

    July 21, 2022 12:44 by Researcher Metadata Database

  • Added Creator Christina Carbrello

    July 21, 2022 12:44 by Researcher Metadata Database

  • Added Creator Sal Giglia

    July 21, 2022 12:44 by Researcher Metadata Database

  • Added Creator Andrew L. Zydney

    July 21, 2022 12:44 by Researcher Metadata Database

  • Published

    July 21, 2022 12:44 by Researcher Metadata Database

  • Updated Work Title, Keyword, Description Show Changes

    November 08, 2022 10:56 by pmk5516

    Work Title
    • Effect of operating pressure on protein fouling during constant-pressure virus removal filtration
    • ! Effect of operating pressure on protein fouling during constant-pressure virus removal filtration
    Keyword
    • Virus filtration, Monoclonal antibody, Bioprocessing, Fouling
    Description
    • <p>Virus removal filtration provides a robust size-based removal of enveloped and non-enveloped viruses in the production of monoclonal antibodies and serum-derived products. The filtrate flux in virus removal filtration is typically dominated by membrane fouling associated with the therapeutic protein product, but very few studies have examined the effect of operating pressure on the fouling rate/mechanisms or the virus filter capacity. Filtration experiments were performed using Viresolve® Pro virus removal filters with human serum Immunoglobulin G (hIgG) as a model protein over a range of operating pressures from 10 to 60 psi. The extent of fouling (evaluated in terms of the overall resistance at a given throughput) decreased with increasing operating pressure up to about 30 psi, while the filter capacity (evaluated using the V<sub>max</sub> model) increased with increasing pressure. The flux decline behavior was shown to be in good agreement with the combined complete pore blockage and cake filtration model, with the pore blockage parameter decreasing with increasing pressure, while the cake filtration parameter increased with increasing pressure. Confocal microscopy was used to identify the location and extent of protein deposition within the membrane cross-section, providing further support for the effects of pressure on both pore blockage and cake resistance. These results provide important insights into the factors controlling protein fouling during virus removal filtration and the influence of operating pressure on the flux decline and filter capacity.</p>
    • Virus removal filtration provides a robust size-based removal of enveloped and non-enveloped viruses in the production of monoclonal antibodies and serum-derived products. The filtrate flux in virus removal filtration is typically dominated by membrane fouling associated with the therapeutic protein product, but very few studies have examined the effect of operating pressure on the fouling rate/mechanisms or the virus filter capacity. Filtration experiments were performed using Viresolve® Pro virus removal filters with human serum Immunoglobulin G (hIgG) as a model protein over a range of operating pressures from 10 to 60 psi. The extent of fouling (evaluated in terms of the overall resistance at a given throughput) decreased with increasing operating pressure up to about 30 psi, while the filter capacity (evaluated using the Vmax model) increased with increasing pressure. The flux decline behavior was shown to be in good agreement with the combined complete pore blockage and cake filtration model, with the pore blockage parameter decreasing with increasing pressure, while the cake filtration parameter increased with increasing pressure. Confocal microscopy was used to identify the location and extent of protein deposition within the membrane cross-section, providing further support for the effects of pressure on both pore blockage and cake resistance. These results provide important insights into the factors controlling protein fouling during virus removal filtration and the influence of operating pressure on the flux decline and filter capacity.
  • Updated Work Title Show Changes

    May 24, 2023 10:38 by pmk5516

    Work Title
    • ! Effect of operating pressure on protein fouling during constant-pressure virus removal filtration
    • Effect of operating pressure on protein fouling during constant-pressure virus removal filtration
  • Updated

    April 04, 2024 10:21 by [unknown user]