Mechanism of eIF6-mediated inhibition of ribosomal subunit joining
During the process of ribosomal assembly, the essential eukaryotic translation initiation factor 6 (eIF6) is known to act as a ribosomal anti-association factor. However, a molecular understanding of the anti-association activity of eIF6 is still missing. Here we present the cryo-electron microscopy reconstruction of a complex of the large ribosomal subunit with eukaryotic eIF6 from Saccharomyces cerevisiae. The structure reveals that the eIF6 binding site involves mainly rpL23 (L14p in Escherichia coli). Based on our structural data, we propose that the mechanism of the anti-association activity of eIF6 is based on steric hindrance of intersubunit bridge formation around the dynamic bridge B6.
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Work Title Mechanism of eIF6-mediated inhibition of ribosomal subunit joining Access Creators - Marco Gartmann
- Michael Blau
- Jean Paul Armache
- Thorsten Mielke
- Maya Topf
- Roland Beckmann
License CC BY 4.0 (Attribution) Work Type Article Publisher - Journal of Biological Chemistry
Publication Date March 31, 2010 Publisher Identifier (DOI) - https://doi.org/10.1074/jbc.C109.096057
Deposited March 07, 2024 Versions
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Added Creator Michael Blau
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Added Creator Jean Paul Armache
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Added Creator Thorsten Mielke
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Added Creator Maya Topf
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Added Creator Roland Beckmann
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2010-05-14- 2010-03-31
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