Assessment of Bone Fracture Healing Using Micro-Computed Tomography
Micro-computed tomography (µCT) is the most common imaging modality to characterize the three-dimensional (3D) morphology of bone and newly formed bone during fracture healing in translational science investigations. Studies of long bone fracture healing in rodents typically involve secondary healing and the formation of a mineralized callus. The shape of the callus formed and the density of the newly formed bone may vary substantially between timepoints and treatments. Whereas standard methodologies for quantifying parameters of intact cortical and trabecular bone are widely used and embedded in commercially available software, there is a lack of consensus on procedures for analyzing the healing callus. The purpose of this work is to describe a standardized protocol that quantitates bone volume fraction and callus mineral density in the healing callus. The protocol describes different parameters that should be considered during imaging and analysis, including sample alignment during imaging, the size of the volume of interest, and the number of slices that are contoured to define the callus.
© MyJove Corporation
Files
Metadata
Work Title | Assessment of Bone Fracture Healing Using Micro-Computed Tomography |
---|---|
Access | |
Creators |
|
License | In Copyright (Rights Reserved) |
Work Type | Article |
Publisher |
|
Publication Date | December 9, 2022 |
Publisher Identifier (DOI) |
|
Deposited | May 06, 2024 |
Versions
Analytics
Collections
This resource is currently not in any collection.