Assessment of Bone Fracture Healing Using Micro-Computed Tomography

Micro-computed tomography (µCT) is the most common imaging modality to characterize the three-dimensional (3D) morphology of bone and newly formed bone during fracture healing in translational science investigations. Studies of long bone fracture healing in rodents typically involve secondary healing and the formation of a mineralized callus. The shape of the callus formed and the density of the newly formed bone may vary substantially between timepoints and treatments. Whereas standard methodologies for quantifying parameters of intact cortical and trabecular bone are widely used and embedded in commercially available software, there is a lack of consensus on procedures for analyzing the healing callus. The purpose of this work is to describe a standardized protocol that quantitates bone volume fraction and callus mineral density in the healing callus. The protocol describes different parameters that should be considered during imaging and analysis, including sample alignment during imaging, the size of the volume of interest, and the number of slices that are contoured to define the callus.

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Work Title Assessment of Bone Fracture Healing Using Micro-Computed Tomography
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Open Access
Creators
  1. Hwa Bok Wee
  2. Deepak Kumar Khajuria
  3. Fadia Kamal
  4. Gregory S. Lewis
  5. Reyad A. Elbarbary
License In Copyright (Rights Reserved)
Work Type Article
Publisher
  1. Journal of Visualized Experiments
Publication Date December 9, 2022
Publisher Identifier (DOI)
  1. https://doi.org/10.3791/64262
Deposited May 06, 2024

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Version 1
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  • Created
  • Added nihms-1908164-1.pdf
  • Added Creator Fadia A Kamal
  • Added Creator Reyad Elbarbary
  • Added Creator Hwa Bok Wee
  • Added Creator H Wee
  • Added Creator Deepak Kumar Khajuri
  • Added Creator D K Khajuria
  • Added Creator Fadia Kamal
  • Added Creator D K Khajuria
  • Added Creator F Kamal
  • Added Creator Gregory Stephen Lewis
  • Added Creator Reyad A Elbarbary
  • Added Creator G Lewis
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  • Deleted Creator H Wee
  • Deleted Creator D K Khajuria
  • Deleted Creator Fadia Kamal
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  • Deleted Creator Reyad A Elbarbary
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    • Reyad Elbarbary
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