Engineering bromodomains with a photoactive amino acid by engaging 'Privileged' tRNA synthetases
Site-specific placement of unnatural amino acids, particularly those responsive to light, offers an elegant approach to control protein function and capture their fleeting 'interactome'. Herein, we have resurrected 4-(trifluoromethyldiazirinyl)-phenylalanine, an underutilized photo-crosslinker, by introducing several key features including easy synthetic access, site-specific incorporation by 'privileged' synthetases and superior crosslinking efficiency, to develop photo-crosslinkable bromodomains suitable for 'interactome' profiling.
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| Work Title | Engineering bromodomains with a photoactive amino acid by engaging 'Privileged' tRNA synthetases |
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| License | In Copyright (Rights Reserved) |
| Work Type | Article |
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| Publication Date | March 28, 2020 |
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| Deposited | April 14, 2025 |
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