Prefiltration enhances performance of sterile filtration for glycoconjugate vaccines

Recent studies have reported very low capacity during sterile filtration of glycoconjugate vaccines due to rapid fouling of the sterile filter. The objective of this study was to explore the potential for significantly increasing the capacity of the sterile filter through the use of an appropriate prefilter. Data were obtained using prefilters with different pore size and chemistry, with the sterile filtration performed at constant filtrate flux using 0.22 μm nominal pore size Durapore® polyvinylidene difluoride membranes. Prefiltration through 5 μm pore size Durapore® or Nylon prefilters nearly eliminated the fouling of the sterile filter, leading to more than a 100-fold reduction in the rate of pressure increase for the sterile filter. This dramatic improvement in sterile filter performance was due to the removal of large components (greater than 1 μm in size) as confirmed by dynamic light scattering. These results demonstrate the potential of using large pore size prefilters to significantly enhance the performance of the sterile filtration process for the production of important glycoconjugate vaccines.

Files

Metadata

Work Title Prefiltration enhances performance of sterile filtration for glycoconjugate vaccines
Access
Open Access
Creators
  1. Zhuoshi Du
  2. Seyed Pouria Motevalian
  3. Brenda Carillo-Conde
  4. Kelvin Reilly
  5. Andrew L. Zydney
License In Copyright (Rights Reserved)
Work Type Article
Publisher
  1. Biotechnology Progress
Publication Date September 1, 2021
Publisher Identifier (DOI)
  1. https://doi.org/10.1002/btpr.3180
Deposited November 23, 2021

Versions

Analytics

Collections

This resource is currently not in any collection.

Work History

Version 1
published

  • Created
  • Added Glycoconjugate_Prefiltration-revised.docx
  • Added Creator Zhuoshi Du
  • Added Creator Seyed Pouria Motevalian
  • Added Creator Brenda Carillo-Conde
  • Added Creator Kelvin Reilly
  • Added Creator Andrew L. Zydney
  • Published
  • Updated
  • Updated
  • Updated