P06_GAATTCGT_raw_merged.fastq.gz

This project studied the effects of thermal stress on gene expression in the Staghorn coral (Acropora cervicornis). At four seasonal time points, tissue from at least ten colonies at three nurseries were exposed to three temperature treatments for one hour then preserved in RNALater. RNA was extracted with TRI Reagent then cleaned with a Qiagen RNEasy kit. A subset of samples were chosen and pooled for RNA-Seq. The subset included genotypes from 2 of the 3 nurseries (Upper Keys and Lower Keys), 2 of the 4 seasonal collections (Summer 1 and Winter 1), and all 3 temperature treatments (ambient, hot, and cold), resulting in 12 libraries. For each library, RNA from 7 of the 10+ genotypes at each nursery were pooled (84 samples total). Following the manufacturer’s protocol, 150 bp single reads were generated from oligo(dT)-selected total RNA using the Illumina TruSeq Stranded mRNA HT Kit. mRNA sequencing libraries were individually barcoded, multiplexed in equal quantities, and run on three lanes of the Illumina HiSeq 2000 platform. The data were demultiplexed into 12 individual library files.

Files

Metadata

Work Title P06_GAATTCGT_raw_merged.fastq.gz
Access
Open Access
Creators
  1. Parkinson, John
  2. Baums, Illiana
Keyword
  1. Acropora cervicornus
  2. functional genomics
  3. Staghorn coral
  4. heat stress
  5. RNA sequencing
License Attribution 3.0 United States
Work Type Dataset
Deposited February 15, 2017

Versions

Analytics

Collections

Work History

Version 1
published

  • Created
  • Added wh702q794r_version1_P06_GAATTCGT_raw_merged.fastq.gz
  • Added Creator Parkinson, John
  • Added Creator Baums, Illiana
  • Published