HCMV infection downregulates GPX4 and stimulates lipid peroxidation but does not induce ferroptosis Figure 1

FIG 1 HCMV differentially regulates GPX4 and FSP1 during infection. (A) Lipid peroxidation measured with C11 BODIPY FITC fluorescence shift in MRC-5 cells either uninfected (0 hpi) or TB40/E-WT (MOI = 3) infected at 24, 48, 72, 96, and 120 hpi. Lipid peroxidation is shown relative to uninfected cells. Results are from two individual experiments, n = 6. (B) Model depicting the pathways of GPX4 and FSP1 to reduce lipid peroxidation and regulate ferroptosis. (C) Western blot analysis of lysates from uninfected MRC5 cells or MRC5 cells infected with TB40/E-WT (as in [A]) and harvested at 24, 48, 72, 96, and 120 hours. Blots were probed for GPX4, FSP1, IE2, pp28, and p115 as a loading control. The values under the GPX4 and FSP1 blots denote quantification of each GPX4 or FSP1 band in the blot, relative to the GPX4 or FSP1 band in the uninfected cells at 24 hours, respectively. For the above panels, * indicates P < 0.05 and ns = not signific

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Norbury, Christopher (2024). HCMV infection downregulates GPX4 and stimulates lipid peroxidation but does not induce ferroptosis Figure 1 [Data set]. Scholarsphere.

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Work Title HCMV infection downregulates GPX4 and stimulates lipid peroxidation but does not induce ferroptosis Figure 1
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  1. Christopher Norbury
License CC BY 4.0 (Attribution)
Work Type Dataset
Publication Date December 20, 2024
Deposited December 20, 2024

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  • Updated Description, Publication Date Show Changes
    Description
    • FIG 1 HCMV differentially regulates GPX4 and FSP1 during infection. (A) Lipid peroxidation measured with C11 BODIPY FITC fluorescence shift in MRC-5 cells either uninfected (0 hpi) or TB40/E-WT (MOI = 3) infected at 24, 48, 72, 96, and 120 hpi. Lipid peroxidation is shown relative to uninfected cells. Results are from two individual experiments, n = 6. (B) Model depicting the pathways of GPX4 and FSP1 to reduce lipid peroxidation and regulate ferroptosis. (C) Western blot analysis of lysates from uninfected MRC5 cells or MRC5 cells infected with TB40/E-WT (as in [A]) and harvested at 24, 48, 72, 96, and 120 hours. Blots were probed for GPX4, FSP1, IE2, pp28, and p115 as a loading control. The values under the GPX4 and FSP1 blots denote quantification of each GPX4 or FSP1 band in the blot, relative to the GPX4 or FSP1 band in the uninfected cells at 24 hours, respectively. For the above panels, * indicates P < 0.05 and ns = not signific
    Publication Date
    • 2024-12-20
  • Added Creator Christopher Norbury
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  • Added 053024 Lipid Assay timecourse data.xpt.xlsx
  • Added 081524 lipid assay timecourse.xlsx
  • Added 082224 Lipid assay timecourse.xlsx
  • Added 082924 Lipid assay timecourse.xlsx
  • Added 090524 lipid assay dilutions.xlsx
  • Added FSP1 Maddie Martin 2024-08-15 18h21m29s.tif
  • Added IE86.D4 Maddie Martin 2024-08-15 13h41m04s.tif
  • Added p115 Maddie Martin 2024-08-16 14h18m55s.tif
  • Added pp28 Maddie Martin 2024-08-16 14h26m08s.tif
  • Added .DS_Store
  • Added GPX4 Maddie Martin 2024-08-16 14h40m40s.tif
  • Added IE86.D4 Maddie Martin 2024-08-15 13h34m03s.tif
  • Added p115 Maddie Martin 2024-08-16 14h35m48s.tif
  • Added UI and TB40WT timecourse westerns protein calcs.xlsx
  • Added 020124 GPX4 OE protein calcs.xlsx
  • Updated License Show Changes
    License
    • https://creativecommons.org/licenses/by/4.0/
  • Added Fig1 readme.txt
  • Added Figure 1.pdf
  • Deleted Fig1 readme.txt
  • Added Fig1 readme.txt
  • Deleted Fig1 readme.txt
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  • Published
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