Regiospecific Cellulose Orientation and Anisotropic Mechanical Property in Plant Cell Walls

Cellulose microfibrils (CMFs) are a major load-bearing component in plant cell walls. Thus, their structures have been studied extensively with spectroscopic and microscopic characterization methods, but the findings from these two approaches were inconsistent, which hampers the mechanistic understanding of cell wall mechanics. Here, we report the regiospecific assembly of CMFs in the periclinal wall of plant epidermal cells. Using sum frequency generation spectroscopic imaging, we found that CMFs are highly aligned in the cell edge region where two cells form a junction, whereas they are mostly isotropic on average throughout the wall thickness in the flat face region of the epidermal cell. This subcellular-level heterogeneity in the CMF alignment provided a new perspective on tissue-level anisotropy in the tensile modulus of cell wall materials. This finding also has resolved a previous contradiction between the spectroscopic and microscopic imaging studies, which paves a foundation for better understanding of the cell wall architecture, especially structure-geometry relationships.

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Work Title Regiospecific Cellulose Orientation and Anisotropic Mechanical Property in Plant Cell Walls
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Open Access
Creators
  1. J Lee
  2. J Choi
  3. L. Feng
  4. J Yu
  5. Y Zheng
  6. Q Zhang
  7. Y Lin
  8. S. Sah
  9. Ying Gu
  10. Sulin Zhang
  11. Daniel J Cosgrove
  12. Seong H. Kim
Keyword
  1. Sum frequency generation (SFG) spectroscopy
  2. cellulose microfibrils
  3. plant cell walls mechanics
  4. finite element analysis (FEA)
  5. biomaterials
License In Copyright (Rights Reserved)
Work Type Article
Publisher
  1. Biomacromolecules
Publication Date September 13, 2023
Publisher Identifier (DOI)
  1. https://doi.org/10.1021/acs.biomac.3c00538
Deposited July 05, 2024

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Version 1
published

  • Created
  • Added CrPS-regiospecific_cellulose_orientation_ms_dc_edits.pdf
  • Added Creator J Lee
  • Added Creator J Choi
  • Added Creator L. Feng
  • Added Creator J Yu
  • Added Creator Y Zheng
  • Added Creator Q Zhang
  • Added Creator Y Lin
  • Added Creator S. Sah
  • Added Creator Ying Gu
  • Added Creator Sulin Zhang
  • Added Creator Daniel J Cosgrove
  • Added Creator Seong H. Kim
  • Published
  • Updated
  • Updated Keyword, Publication Date Show Changes
    Keyword
    • Sum frequency generation (SFG) spectroscopy, cellulose microfibrils, plant cell walls mechanics, finite element analysis (FEA), biomaterials
    Publication Date
    • 2023-01-01
    • 2023-09-13
  • Deleted CrPS-regiospecific_cellulose_orientation_ms_dc_edits.pdf
  • Added Regiospecific cellulose orientation_ma.pdf
  • Added Regiospecific cellulose orientation_si.pdf.pdf