Skin-interfaced microfluidic devices with one-opening chambers and hydrophobic valves for sweat collection and analysis

Soft, skin-interfaced microfluidic platforms are capable of capturing, storing, and assessing sweat chemistry and total sweat loss, which provides essential insight into human physiological health. However, sweat loss from the outlet of the microfluidic devices often leads to deviation of the measured concentration of the biomarker or electrolyte from the actual value. Here, we introduce hydrophobic valves at the junction of the chamber and the microfluidic channel as a new chamber design to reduce sweat evaporation. Because the advancing front of the liquid in the hydrophilic microchannel is blocked by the hydrophobic valve, the fluid flows into the chambers, forms the initial meniscus, and completely fills the chambers along the initial meniscus. Fluid dynamic modeling and numerical simulations provide critical insights into the sweat sampling mechanism into the chambers. With significantly reduced evaporation and contamination, the sweat sample can be easily stored for a long time for later analysis when in situ analysis is limited. Additionally, the design with multiple chambers can allow sequential generation of sweat collection at different times for long-term analysis. The in situ real-time measurements of the sweat loss and pH value analysis from the human subject demonstrate the practical utility of the devices in collecting, storing, and analyzing the sweat generated from sweat glands on the skin.

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Work Title Skin-interfaced microfluidic devices with one-opening chambers and hydrophobic valves for sweat collection and analysis
Access
Open Access
Creators
  1. Yingxue Zhang
  2. Yao Chen
  3. Jielong Huang
  4. Yangchengyi Liu
  5. Jinfeng Peng
  6. Shangda Chen
  7. Kui Song
  8. Xiaoping Ouyang
  9. Huanyu Cheng
  10. Xiufeng Wang
Keyword
  1. Sweat collection and analysis
  2. Hydrophobic valves
  3. Chambers with one opening
  4. Chrono-sampling
License In Copyright (Rights Reserved)
Work Type Article
Publisher
  1. Lab on a Chip
Publication Date June 18, 2020
Publisher Identifier (DOI)
  1. https://doi.org/10.1039/d0lc00400f
Deposited February 17, 2023

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Work History

Version 1
published

  • Created
  • Added LC-ART-04-2020-000400.R1_Proof_hi.pdf
  • Added Creator Yingxue Zhang
  • Added Creator Yao Chen
  • Added Creator Jielong Huang
  • Added Creator Yangchengyi Liu
  • Added Creator Jinfeng Peng
  • Added Creator Shangda Chen
  • Added Creator Kui Song
  • Added Creator Xiaoping Ouyang
  • Added Creator Huanyu Cheng
  • Added Creator Xiufeng Wang
  • Published
  • Updated Keyword, Publisher, Description, and 1 more Show Changes
    Keyword
    • Sweat collection and analysis, Hydrophobic valves, Chambers with one opening, Chrono-sampling
    Publisher
    • Lab on a Chip - Miniaturisation for Chemistry and Biology
    • Lab on a Chip
    Description
    • <p>Soft, skin-interfaced microfluidic platforms are capable of capturing, storing, and assessing sweat chemistry and total sweat loss, which provides essential insight into human physiological health. However, sweat loss from the outlet of the microfluidic devices often leads to deviation of the measured concentration of the biomarker or electrolyte from the actual value. Here, we introduce hydrophobic valves at the junction of the chamber and the microfluidic channel as a new chamber design to reduce sweat evaporation. Because the advancing front of the liquid in the hydrophilic microchannel is blocked by the hydrophobic valve, the fluid flows into the chambers, forms the initial meniscus, and completely fills the chambers along the initial meniscus. Fluid dynamic modeling and numerical simulations provide critical insights into the sweat sampling mechanism into the chambers. With significantly reduced evaporation and contamination, the sweat sample can be easily stored for a long time for later analysis when in situ analysis is limited. Additionally, the design with multiple chambers can allow sequential generation of sweat collection at different times for long-term analysis. The in situ real-time measurements of the sweat loss and pH value analysis from the human subject demonstrate the practical utility of the devices in collecting, storing, and analyzing the sweat generated from sweat glands on the skin. This journal is </p>
    • <p>Soft, skin-interfaced microfluidic platforms are capable of capturing, storing, and assessing sweat chemistry and total sweat loss, which provides essential insight into human physiological health. However, sweat loss from the outlet of the microfluidic devices often leads to deviation of the measured concentration of the biomarker or electrolyte from the actual value. Here, we introduce hydrophobic valves at the junction of the chamber and the microfluidic channel as a new chamber design to reduce sweat evaporation. Because the advancing front of the liquid in the hydrophilic microchannel is blocked by the hydrophobic valve, the fluid flows into the chambers, forms the initial meniscus, and completely fills the chambers along the initial meniscus. Fluid dynamic modeling and numerical simulations provide critical insights into the sweat sampling mechanism into the chambers. With significantly reduced evaporation and contamination, the sweat sample can be easily stored for a long time for later analysis when in situ analysis is limited. Additionally, the design with multiple chambers can allow sequential generation of sweat collection at different times for long-term analysis. The in situ real-time measurements of the sweat loss and pH value analysis from the human subject demonstrate the practical utility of the devices in collecting, storing, and analyzing the sweat generated from sweat glands on the skin. </p>
    Publication Date
    • 2020-08-07
    • 2020-06-18
  • Deleted LC-ART-04-2020-000400.R1_Proof_hi.pdf
  • Added Lab Chip-Manuscript.docx
  • Updated