Experimental detection of Immunoglobulin G by prism-coupled angular interrogation and a support vector machine

A typical prism-coupled surface-plasmon-resonance biosensor comprises a metal thin film in contact with a solution containing an analyte to be sensed. The metal film also acts as a binding surface for bioreceptor molecules to capture and concentrate the analyte molecules of interest. We investigated the use of a porous, anisotropic, periodically non-homogeneous material called a chiral sculptured thin film (CSTF) grown on top of the metal film to confine the solution in its pores. The efficacy of a basic plasmonic sensor was compared with those of two types of sensors containing a CSTF, one type having a metal-nanoparticle layer at a distance of one period from the metal film and the other without that layer. The chosen analyte was Immunoglobulin G and the chosen bioreceptor was Protein A. Measurements were made over a wide angular range rather than over a small range tied to the excitation of a surface-plasmon-polariton wave, and the collected data were used to train a machine-learning algorithm called a support vector machine for classification. We concluded that the metal/CSTF sensor with a metal-nanoparticle layer performs best, the metal-nanoparticle layer being crucial to its better performance.


  • McAtee_21_1v15A.pdf

    size: 18 MB | mime_type: application/pdf | date: 2022-07-26 | sha256: 1caa884


Work Title Experimental detection of Immunoglobulin G by prism-coupled angular interrogation and a support vector machine
Open Access
  1. Patrick D. McAtee
  2. Akhlesh Lakhtakia
  1. Biosensing
  2. Chiral sculptured thin film
  3. Machine learning
  4. Prism-coupled configuration
  5. Support vector machine
  6. Surface plasmon resonance
License In Copyright (Rights Reserved)
Work Type Article
  1. Journal of Nanophotonics
Publication Date January 8, 2022
Publisher Identifier (DOI)
  1. https://doi.org/10.1117/1.JNP.16.016003
Deposited July 26, 2022




This resource is currently not in any collection.

Work History

Version 1

  • Created
  • Added McAtee_21_1v15A.pdf
  • Added Creator Patrick D. McAtee
  • Added Creator Akhlesh Lakhtakia
  • Published
  • Updated Keyword, Publication Date Show Changes
    • Biosensing, Chiral sculptured thin film, Machine learning, Prism-coupled configuration, Support vector machine, Surface plasmon resonance
    Publication Date
    • 2022-01-01
    • 2022-01-08
  • Updated