Digital electrical impedance analysis for single bacterium sensing and antimicrobial susceptibility testing

Single-molecule and single-cell analysis techniques have opened new opportunities for characterizing and analyzing heterogeneity within biological samples. These detection methods are often referred to as digital assays because the biological sample is partitioned into many small compartments and each compartment contains a discrete number of targets (e.g.cells). Using digital assays, researchers can precisely detect and quantify individual targets, and this capability has made digital techniques the basis for many modern bioanalytical tools (including digital PCR, single cell RNA sequencing, and digital ELISA). However, digital assays are dominated by optical analysis systems that typically utilize microscopy to analyze partitioned samples. The utility of digital assays may be dramatically enhanced by implementing cost-efficient and portable electrical detection capabilities. Herein, we describe a digital electrical impedance sensing platform that enables direct multiplexed measurement of single cell bacterial cells. We outline our solutions to the challenge of multiplexing impedance sensing across many culture compartments and demonstrate the potential for rapidly differentiating antimicrobial resistantversussusceptible strains of bacteria.


  • manuscript_-_PW.docx

    size: 8.54 MB | mime_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document | date: 2021-07-15


Work Title Digital electrical impedance analysis for single bacterium sensing and antimicrobial susceptibility testing
Open Access
  1. Brian Scherer
  2. Christine Surrette
  3. Hui Li
  4. Peter Torab
  5. Erik Kvam
  6. Craig Galligan
  7. Steven Go
  8. Greg Grossmann
  9. Tyler Hammond
  10. Tammy Johnson
  11. Richard St-Pierre
  12. John R. Nelson
  13. Radislav A. Potyrailo
  14. Tejas Khire
  15. Kuangwen Hsieh
  16. Tza Huei Wang
  17. Pak Kin Wong
  18. Chris M. Puleo
License In Copyright (Rights Reserved)
Work Type Article
  1. Lab on a Chip - Miniaturisation for Chemistry and Biology
Publication Date March 21, 2021
Publisher Identifier (DOI)
Deposited July 15, 2021




This resource is currently not in any collection.

Work History

Version 1

  • Created
  • Added manuscript_-_PW.docx
  • Added Creator Brian Scherer
  • Added Creator Christine Surrette
  • Added Creator Hui Li
  • Added Creator Peter Torab
  • Added Creator Erik Kvam
  • Added Creator Craig Galligan
  • Added Creator Steven Go
  • Added Creator Greg Grossmann
  • Added Creator Tyler Hammond
  • Added Creator Tammy Johnson
  • Added Creator Richard St-Pierre
  • Added Creator John R. Nelson
  • Added Creator Radislav A. Potyrailo
  • Added Creator Tejas Khire
  • Added Creator Kuangwen Hsieh
  • Added Creator Tza Huei Wang
  • Added Creator Pak Kin Wong
  • Added Creator Chris M. Puleo
  • Published
  • Updated
  • Updated