Data for "Motor Clustering Enhances Kinesin-driven Vesicle Transport"
Intracellular vesicles are typically transported by a small number of kinesin and dynein motors. However, the slow microtubule binding rate of kinesin-1 observed in in vitro biophysical studies suggests that long-range transport may require a high number of motors. To address the discrepancy in motor requirements between in vivo and in vitro studies, we reconstituted motility of 120-nm-diameter liposomes driven by multiple GFP-labeled kinesin-1 motors. Consistent with predictions based on previous binding rate measurements, we found that long-distance transport requires a high number of kinesin-1 motors. We hypothesized that this discrepancy from in vivo observations may arise from differences in motor organization and tested whether motor clustering can enhance transport efficiency using a DNA scaffold. Clustering just three motors increased liposome travel distances across a wide range of motor numbers. Our findings demonstrate that, independent of motor number, the arrangement of motors on a vesicle regulates transport distance, suggesting that differences in motor organization may explain the disparity between in vivo and in vitro motor requirements for long-range transport.
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| Work Title | Data for "Motor Clustering Enhances Kinesin-driven Vesicle Transport" |
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| License | CC BY-NC 4.0 (Attribution-NonCommercial) |
| Work Type | Dataset |
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| Publication Date | May 5, 2025 |
| DOI | doi:10.26207/becz-me75 |
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| Deposited | May 01, 2025 |
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