Ex vivo LPS-stimulated cytokine production is associated with hydration status in community-dwelling middle-to-older-aged adults

Purpose: Suboptimal hydration has been linked to a variety of adverse health outcomes. Few studies have examined the impact of hydration status on immune function, a plausible physiological mechanism underlying these associations. Therefore, we tested how variation in hydration status was associated with circulating pro-inflammatory cytokine levels and ex vivo lipopolysaccharide (LPS)-stimulated pro-inflammatory cytokine production. Methods: Blood samples were obtained from a community sample of healthy middle-to-older-aged adults (N = 72). These samples were used to assess serum osmolality, a biomarker of hydration status, and markers of immune function including circulating pro-inflammatory cytokines and stimulated pro-inflammatory cytokine production after 4 and 24 h of incubation with LPS. Multiple linear regressions were used to test the association between serum osmolality (as a continuous variable) and markers of immune function at baseline and after 4 and 24 h adjusting for age, sex, and BMI. These models were re-estimated with serum osmolality dichotomized at the cut-off for dehydration (> 300 mOsm/kg). Results: While not significantly associated with circulating cytokines (B = − 0.03, p = 0.09), serum osmolality was negatively associated with both 4 h (B = − 0.05, p = 0.048) and 24 h (B = − 0.05, p = 0.03) stimulated cytokine production when controlling for age, sex, and BMI. Similarly, dehydration was associated with significantly lower cytokine production at both 4 h (B = − 0.54, p = 0.02) and 24 h (B = − 0.51, p = 0.02) compared to adequate hydration. Conclusion: These findings suggest that dehydration may be associated with suppressed immune function in generally healthy middle-to-older aged community-dwelling adults. Further longitudinal research is needed to more clearly define the role of hydration in immune function.

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Work Title Ex vivo LPS-stimulated cytokine production is associated with hydration status in community-dwelling middle-to-older-aged adults
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Open Access
Creators
  1. Kristin M. Davis
  2. Asher Y. Rosinger
  3. Kyle W. Murdock
Keyword
  1. Hydration
  2. Serum osmolality
  3. Inflammation
  4. Ex vivo LPS-stimulated cytokine production
License In Copyright (Rights Reserved)
Work Type Article
Publisher
  1. European Journal of Nutrition
Publication Date February 15, 2023
Publisher Identifier (DOI)
  1. https://doi.org/10.1007/s00394-023-03105-z
Deposited June 20, 2023

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Version 1
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  • Created
  • Added Davis_et_al__2023__Ex_vivo_LPS-stimulated_cytokine_production_is_associated_with_hydration_status_in_community-dwelling_middle-to-older-aged_adults.pdf
  • Added Creator Kristin M. Davis
  • Added Creator Asher Y. Rosinger
  • Added Creator Kyle W. Murdock
  • Published
  • Updated Keyword, Publisher, Publication Date Show Changes
    Keyword
    • Hydration,  Serum osmolality, Inflammation,  Ex vivo LPS-stimulated cytokine production
    Publisher
    • Zeitschrift fur Ernahrungswissenschaft
    • European Journal of Nutrition
    Publication Date
    • 2023-06-01
    • 2023-02-15
  • Updated Keyword Show Changes
    Keyword
    • Hydration,  Serum osmolality, Inflammation,  Ex vivo LPS-stimulated cytokine production
    • Hydration, Serum osmolality, Inflammation, Ex vivo LPS-stimulated cytokine production
  • Updated