! The first Mycoplasma ovipneumoniae recovered from a sheep with respiratory disease in Brazil – draft genome and genomic analysis

Mycoplasma ovipneumoniae is an important etiological agent of sheep respiratory disease worldwide. Here, we describe the first isolation and draft genome sequence of M. ovipneumoniae strain USP-BR2017 retrieved from tracheobronchial lavage of a sheep showing clinical signs of respiratory disease in the Rio de Janeiro State, Brazil. The culture of tracheobronchial lavage resulted in glucose-fermenting fried egg colonies, which were identified as M. ovipneumoniae by polymerase chain reaction. The genome was sequenced using the Illumina NextSeq 2000 and de novo assembled using SPAdes. The genome of the sequenced organism presented an approximate size of 1,122,253 bp. The annotation revealed 773 coding DNA sequences (CDSs), 806 genes, three rRNAs, and 30 tRNAs. Data analysis revealed M. ovipneumoniae strain USP-BR2017 contains a few virulence genes, including the hemolysing C gene (hlyC). In addition, strain USP-BR2017 showed high identity over the 16S rRNA gene with other sheep isolates from China and United States. This first description of M. ovipneumoniae in diseased Brazilian sheep demonstrates the importance of continuous surveillance and diagnostics of pathogens causing respiratory disease in sheep in Brazil.

This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s11259-022-09972-x

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Work Title ! The first Mycoplasma ovipneumoniae recovered from a sheep with respiratory disease in Brazil – draft genome and genomic analysis
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Open Access
Creators
  1. Natália C. Gaeta
  2. Ana Marcia de Sá Guimarães
  3. Jorge Timenetsky
  4. Stephanie Clouser
  5. Lilian Gregory
  6. Erika Ganda
Keyword
  1. Ovine respiratory disease
  2. Pneumonia
  3. Mollicutes
  4. Whole Genome Sequencing
License In Copyright (Rights Reserved)
Work Type Article
Publisher
  1. Veterinary Research Communications
Publication Date July 8, 2022
Publisher Identifier (DOI)
  1. https://doi.org/10.1007/s11259-022-09972-x
Deposited November 07, 2022

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  • Created
  • Added ShortCommunication_Gaetaetal_authorversion.docx
  • Added Creator Natália C. Gaeta
  • Added Creator Ana Marcia de Sá Guimarães
  • Added Creator Jorge Timenetsky
  • Added Creator Stephanie Clouser
  • Added Creator Lilian Gregory
  • Added Creator Erika Ganda
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  • Updated Work Title, Keyword, Description Show Changes
    Work Title
    • The first Mycoplasma ovipneumoniae recovered from a sheep with respiratory disease in Brazil – draft genome and genomic analysis
    • ! The first Mycoplasma ovipneumoniae recovered from a sheep with respiratory disease in Brazil – draft genome and genomic analysis
    Keyword
    • Ovine respiratory disease, Pneumonia, Mollicutes, Whole Genome Sequencing
    Description
    • <p>Mycoplasma ovipneumoniae is an important etiological agent of sheep respiratory disease worldwide. Here, we describe the first isolation and draft genome sequence of M. ovipneumoniae strain USP-BR2017 retrieved from tracheobronchial lavage of a sheep showing clinical signs of respiratory disease in the Rio de Janeiro State, Brazil. The culture of tracheobronchial lavage resulted in glucose-fermenting fried egg colonies, which were identified as M. ovipneumoniae by polymerase chain reaction. The genome was sequenced using the Illumina NextSeq 2000 and de novo assembled using SPAdes. The genome of the sequenced organism presented an approximate size of 1,122,253 bp. The annotation revealed 773 coding DNA sequences (CDSs), 806 genes, three rRNAs, and 30 tRNAs. Data analysis revealed M. ovipneumoniae strain USP-BR2017 contains a few virulence genes, including the hemolysing C gene (hlyC). In addition, strain USP-BR2017 showed high identity over the 16S rRNA gene with other sheep isolates from China and United States. This first description of M. ovipneumoniae in diseased Brazilian sheep demonstrates the importance of continuous surveillance and diagnostics of pathogens causing respiratory disease in sheep in Brazil.</p>
    • Mycoplasma ovipneumoniae is an important etiological agent of sheep respiratory disease worldwide. Here, we describe the first isolation and draft genome sequence of M. ovipneumoniae strain USP-BR2017 retrieved from tracheobronchial lavage of a sheep showing clinical signs of respiratory disease in the Rio de Janeiro State, Brazil. The culture of tracheobronchial lavage resulted in glucose-fermenting fried egg colonies, which were identified as M. ovipneumoniae by polymerase chain reaction. The genome was sequenced using the Illumina NextSeq 2000 and de novo assembled using SPAdes. The genome of the sequenced organism presented an approximate size of 1,122,253 bp. The annotation revealed 773 coding DNA sequences (CDSs), 806 genes, three rRNAs, and 30 tRNAs. Data analysis revealed M. ovipneumoniae strain USP-BR2017 contains a few virulence genes, including the hemolysing C gene (hlyC). In addition, strain USP-BR2017 showed high identity over the 16S rRNA gene with other sheep isolates from China and United States. This first description of M. ovipneumoniae in diseased Brazilian sheep demonstrates the importance of continuous surveillance and diagnostics of pathogens causing respiratory disease in sheep in Brazil.
  • Updated