OpenArray_Design

A spreadsheet containing two workbooks related to the OpenArray high-throughput qPCR assay design for the coral nursery gene expression project. The first workbook includes Assay Number (1-28), OpenArray ID (a short name for the assay generated during array design), Plate ID (a longer name to connect assay to genbank sequences), Publication Name (the name of the gene used in the manuscript, which is different from that used in array design), Species (host Acropora cervicornis or symbiont Symbiodinium sp.), Category (synergism, antagonsim, etc.), Expected Expression Pattern (based on RNAseq analysis), Genbank Accession, Uniprot Accession, Gene Abbreviation, and Gene Description. The second workbook shows the physical layout of the assays on the OpenArray plate. Note that though there were 28 targets, they were printed in duplicate on the plate, providing technical replication and a total assay count of 56.

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Work Title OpenArray_Design
Access
Open Access
Creators
  1. Parkinson, John E
  2. Baums, Iliana B
Keyword
  1. target
  2. htqpcr
  3. assay
  4. design
  5. qpcr
  6. plate
  7. openarray
  8. gene
License Attribution 3.0 United States
Work Type Dataset
Publisher
  1. Penn State University
Publication Date September 29, 2016
Language
  1. English
Deposited September 29, 2016

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Version 1
published

  • Created
  • Added 9cn69m430x_version1_parkinson_openarray_design.xls
  • Added Creator Parkinson, John E
  • Added Creator Baums, Iliana B
  • Published