Inter-specific hybridization of Alnus rubra and Alnus rhombifolia

Exploratory hybridization of red alder (Alnus rubra Bong.) and white alder (Alnus rhombifolia Nutt.) was conducted to initiate a long-term investigation into the inter-specific taxon’s potential for biomass production. Red alder selections originated in stands west of Oregon’s Cascade Mountains, while white alder came from provenances west and east of the Cascades. White alder’s heretofore unknown chromosome number was determined equal to red alder (2n = 2x = 28) and included a pair of satellite chromosomes. An experimental crossing technique was proven, establishing the species’ reproductive affinity for the first time. Hybridization was substantially more productive when red alder served as the female parent as opposed to white alder. Hybridity was confirmed using four species-specific SSR markers developed from transcriptome resources for identifying sib relationships in domesticated populations. To begin a description of the inter-specific taxon, hybrids and their open-pollinated siblings were assessed at ages four through six for morphological and phenological traits and carbon and nitrogen isotopic ratios in a field planting in western Washington, USA. Principal component analysis of quantitative leaf and internode morphologies showed that red alder and hybrid alder are relatively comparable and both are distinct from white alder. Qualitative differences in leaf morphology are more diagnostic with inter-specific hybrids often intermediate to red and white alder. Principal coordinate analysis of molecular data cleanly separated the three taxa. Plans for continued investigation of the taxon as a biomass crop species are discussed including a potential role for inter-specific hybrids in adapting red alder to the effects of climate change.

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Work Title Inter-specific hybridization of Alnus rubra and Alnus rhombifolia
Subtitle preliminary report of a new taxon and DNA marker resources for bioenergy feedstock production
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Open Access
Creators
  1. Brian J. Stanton
  2. Teodora Best
  3. Nurul Islam-Faridi
  4. Carlos Gantz
  5. Kathy Haiby
  6. Lianna J. Johnson
  7. Rich Shuren
  8. Alex Stanish
  9. Margaret Staton
  10. T. Casey Weathers
  11. Di Wu
  12. John E. Carlson
License In Copyright (Rights Reserved)
Work Type Article
Publisher
  1. Tree Genetics and Genomes
Publication Date October 1, 2020
Publisher Identifier (DOI)
  1. https://doi.org/10.1007/s11295-020-01457-9
Deposited November 15, 2021

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  • Created
  • Added Stanton2020_Article_Inter-specificHybridizationOfA.pdf
  • Added 11295_2020_1457_MOESM1_ESM.pdf
  • Added Creator Brian J. Stanton
  • Added Creator Teodora Best
  • Added Creator Nurul Islam-Faridi
  • Added Creator Carlos Gantz
  • Added Creator Kathy Haiby
  • Added Creator Lianna J. Johnson
  • Added Creator Rich Shuren
  • Added Creator Alex Stanish
  • Added Creator Margaret Staton
  • Added Creator T. Casey Weathers
  • Added Creator Di Wu
  • Added Creator John E. Carlson
  • Published
  • Updated
  • Updated