Short communication: Short-term effect of 3-nitrooxypropanol on feed dry matter intake in lactating dairy cows

The objective of this study was to investigate the effect of 3-nitrooxypropanol (3-NOP), an enteric methane inhibitor under investigation, on short-term dry matter intake (DMI) in lactating dairy cows. Following a 1-wk adaptation period, 12 multiparous Holstein cows were fed a basal total mixed ration (TMR) containing increasing levels of 3-NOP during 5 consecutive, 6-d periods. The experiment was conducted in a tiestall barn. Feed bins were split in half by a solid divider, and cows simultaneously received the basal TMR supplemented with the following: (1) a placebo without 3-NOP or (2) 3-NOP included in the TMR at 30, 60, 90, or 120 mg/ kg of feed dry matter (experimental periods 2, 3, 4, and 5, respectively). Cows received the control diet (basal TMR plus placebo premix) during experimental period 1. A premix containing ground corn grain, soybean oil, and dry molasses was used to incorporate 3-NOP in the ration. Cows were fed twice daily as follows: 60% of the daily feed allowance at 0800 h and 40% at 1800 h. Feed offered and refused was recorded at each feeding. During the morning feedings, each cow was offered either control or 3-NOP-treated TMR at 150% of her average intake during the previous 3 d. After collection of the evening refusals, cows received only the basal TMR without the premix until the next morning feeding. The test period for the short-term DMI data collection was defined from morning feeding to afternoon refusals collection during each day of each experimental period. Location (left or right) of the control and 3-NOP diets within a feed bin was switched every day during each period to avoid feed location bias. Dry matter intake of TMR during the test period was quadratically increased by 3-NOP compared with the control. Inclusion of 3-NOP at 120 mg/kg of feed dry matter resulted in decreased 10-h DMI compared with the lower 3-NOP doses, but was similar to the control. There was no effect of feed location (left or right) within feed bin on DMI. Data from this short-term study suggests that 3-NOP does not have a negative effect on DMI in lactating dairy cows.



Work Title Short communication: Short-term effect of 3-nitrooxypropanol on feed dry matter intake in lactating dairy cows
Open Access
  1. Audino Melgar Moreno
  2. Krum Nedelkov
  3. Susanna Raeisaenen
  4. Cristian Martins
  5. Katieli Welter
  6. Xianjiang Chen
  7. Michael Harper
  9. Stephane Duval
  10. Alexander Hristov
  1. 3-nitrooxypropanol
  2. short-term dry matter intake
  3. dairy cattle
License CC BY-NC-ND 4.0 (Attribution-NonCommercial-NoDerivatives)
Work Type Article
  1. Elsevier Inc
  2. Fass Inc
Publication Date June 29, 2020
Publisher Identifier (DOI)
  1. 10.3168/jds.2020-18331
Deposited March 09, 2021




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Work History

Version 1

  • Created
  • Added Creator Audino Melgar Moreno
  • Added Creator Krum Nedelkov
  • Added Creator Susanna Raeisaenen
  • Added Creator Cristian Martins
  • Added Creator Katieli Welter
  • Added Creator Xianjiang Chen
  • Added Creator Michael Harper
  • Added Creator JOONPYO OH
  • Added Creator Stephane Duval
  • Added Creator Alexander Hristov
  • Added Melgar et al. 2020 Short communication 3NOP palatability.pdf
  • Updated License Show Changes
  • Published
  • Updated
  • Updated