Effects of E-Cigarette Aerosols with Varying Levels of Nicotine on Biomarkers of Oxidative Stress and Inflammation in Mice

To provide insights into the cause of e-cigarette (e-cig) associated lung injury, we examined the effects of propylene glycol (PG) and glycerol (G), two common solvent carriers used to deliver nicotine/flavor, on markers of oxidative stress and inflammation in female B6C3F1 mice which had been used successfully in tobacco smoke (TS)-induced lung carcinogenesis. Mice exposed to air and TS were used as negative and positive controls, respectively. Using LC-MS/MS, we showed that PG/G alone, in the absence of nicotine, significantly increased the levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG or its tautomer 8-oxodG), a biomarker of DNA oxidative damage, in lung and plasma of mice; moreover, addition of nicotine (12 and 24 mg/mL) in e-cig liquid appears to suppress the levels of 8-oxodG. Exposure to e-cig aerosols or TS induced nonsignificant increases of plasma C-reactive protein (CRP), a biomarker of inflammation; nonetheless, the levels of fibronectin (FN), a biomarker of tissue injury, were significantly increased by e-cig aerosols or TS. Although preliminary, our data showed that exposure to e-cig aerosols induced a higher score of lung injury than did control air or TS exposure. Our results indicate that the B6C3F1 mouse model may be suitable for an in-depth examination of the impact of e-cig on lung injury associated with oxidative stress and inflammation and this study adds to the growing evidence that the use of e-cig can lead to lung damage.

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Work Title Effects of E-Cigarette Aerosols with Varying Levels of Nicotine on Biomarkers of Oxidative Stress and Inflammation in Mice
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Open Access
Creators
  1. Yuan-Wan Sun
  2. Kun-Ming Chen
  3. Hannah Atkins
  4. Cesar Aliaga
  5. Terry Gordon
  6. Joseph B. Guttenplan
  7. Karam El-Bayoumy
License In Copyright (Rights Reserved)
Work Type Article
Publisher
  1. Chemical Research in Toxicology
Publication Date March 24, 2021
Publisher Identifier (DOI)
  1. https://doi.org/10.1021/acs.chemrestox.1c00033
Deposited July 20, 2022

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Version 1
published

  • Created
  • Added acs.chemrestox.1c00033.pdf
  • Added Creator Yuan Wan Sun
  • Added Creator Kun Ming Chen
  • Added Creator Hannah Atkins
  • Added Creator Cesar Aliaga
  • Added Creator Terry Gordon
  • Added Creator Joseph B. Guttenplan
  • Added Creator Karam El-Bayoumy
  • Published
  • Updated Publication Date Show Changes
    Publication Date
    • 2021-04-19
    • 2021-03-24
  • Renamed Creator Yuan-Wan Sun Show Changes
    • Yuan Wan Sun
    • Yuan-Wan Sun
  • Renamed Creator Kun-Ming Chen Show Changes
    • Kun Ming Chen
    • Kun-Ming Chen