Evaluating Nanoparticle Hydrophobicity Using Analytical Membrane Hydrophobic Interaction Chromatography

Nanoparticle hydrophobicity is a key factor controlling the stability, adhesion, and transport of nanoparticle suspensions. Although a number of approaches have been presented for evaluating nanoparticle hydrophobicity, these methods are difficult to apply to larger nanoparticles and viruses (>100 nm in size) that are of increasing importance in drug delivery and gene therapy. This study investigated the use of a new analytical hydrophobic interaction chromatography method employing a 5.0 µm pore size polyvinylidene fluoride membrane as the stationary phase (MHIC). Experimental data obtained using a series of model proteins were in good agreement with literature values for the hydrophobicity (both experimental and computational). MHIC was then used to evaluate the hydrophobicity of a variety of nanoparticles, including a live attenuated viral vaccine, both in water and in the presence of different surfactants. This new method can be implemented on any liquid chromatography system, run times are typically <20 min, and the experiments avoid the use of organic solvents that could alter the structure of many biological nanoparticles.



Work Title Evaluating Nanoparticle Hydrophobicity Using Analytical Membrane Hydrophobic Interaction Chromatography
Open Access
  1. Neil Taylor
  2. Wanli Ma
  3. Adam Kristopeit
  4. Sheng-ching Wang
  5. Andrew Zydney
  1. Nanoparticle
  2. Hydrophobicity
  3. Virus
  4. Vaccine
  5. Chromatography
License CC BY-NC-ND 4.0 (Attribution-NonCommercial-NoDerivatives)
Work Type Article
  1. Analytical Chemistry
Publication Date June 8, 2022
Publisher Identifier (DOI)
  1. https://doi.org/10.1021/acs.analchem.2c00710
Deposited January 23, 2023




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Work History

Version 1

  • Created
  • Added Evaluating_nanoparticle_hydrophobicity.pdf
  • Added Creator Neil Taylor
  • Added Creator Wanli Ma
  • Added Creator Adam Kristopeit
  • Added Creator Sheng-ching Wang
  • Added Creator Andrew Zydney
  • Published
  • Updated Keyword, Publication Date Show Changes
    • Nanoparticle, Hydrophobicity, Virus, Vaccine, Chromatography
    Publication Date
    • 2022-06-21
    • 2022-06-08
  • Updated