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Cocoa and Polyphenol-Rich Cocoa Fractions Fail to Improve Acute Colonic Inflammation in Dextran Sulfate Sodium-Treated Mice

Scope: A study is conducted to determine the anti-inflammatory effects of cocoa and polyphenol-rich cocoa fractions in the dextran sulfate sodium (DSS)-induced mouse model of acute colonic inflammation.

Methods and results: Male C57BL/6J mice are treated with dietary cocoa powder, an extractable cocoa polyphenol fraction, or a non-extractable cocoa polyphenol fraction for 2 weeks prior to treatment with 2.5% DSS in the drinking water for 7 days to induce colonic inflammation. Cocoa treatment continues during the DSS period. Cocoa and/or cocoa fractions exacerbate DSS-induced weight loss and fail to mitigate DSS-induced colon shortening but do improve splenomegaly. Cocoa/cocoa fraction treatment fails to mitigate DSS-induced mRNA and protein markers of inflammation. Principal component analysis shows overlap between cocoa or cocoa fraction-treated mice and DSS-induced controls, but separation from mice not treated with DSS.

Conclusion: The results suggest cocoa and cocoa polyphenols may not be useful in mitigating acute colonic inflammation.

This is the peer reviewed version of the following article: [Cocoa and Polyphenol‐Rich Cocoa Fractions Fail to Improve Acute Colonic Inflammation in Dextran Sulfate Sodium‐Treated Mice. Molecular Nutrition & Food Research 68, 15 (2024)], which has been published in final form at https://doi.org/10.1002/mnfr.202400431. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions: https://authorservices.wiley.com/author-resources/Journal-Authors/licensing/self-archiving.html#3.

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Work Title Cocoa and Polyphenol-Rich Cocoa Fractions Fail to Improve Acute Colonic Inflammation in Dextran Sulfate Sodium-Treated Mice
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Embargoed
Creators
  1. Daphne K. Weikart
  2. Kiana M. Coleman
  3. Michael G. Sweet
  4. Ashley M. McAmis
  5. Helene Hopfer
  6. Andrew P. Neilson
  7. Joshua D. Lambert
Keyword
  1. Cocoa
  2. Polyphenols
  3. Dextran sulfate sodium
  4. Inflammation
  5. Ulcerative colitis
License In Copyright (Rights Reserved)
Work Type Article
Publisher
  1. Molecular Nutrition and Food Research
Publication Date July 4, 2024
Publisher Identifier (DOI)
  1. https://doi.org/10.1002/mnfr.202400431
Deposited February 24, 2025

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Version 1
published

  • Created

    February 24, 2025 08:53 by Researcher Metadata Database

  • Added mnfr_resubmit_2024061.pdf

    February 24, 2025 08:53 by Researcher Metadata Database

  • Added Creator Daphne K. Weikart

    February 24, 2025 08:53 by Researcher Metadata Database

  • Added Creator Kiana M. Coleman

    February 24, 2025 08:53 by Researcher Metadata Database

  • Added Creator Michael G. Sweet

    February 24, 2025 08:53 by Researcher Metadata Database

  • Added Creator Ashley M. McAmis

    February 24, 2025 08:53 by Researcher Metadata Database

  • Added Creator Helene Hopfer

    February 24, 2025 08:53 by Researcher Metadata Database

  • Added Creator Andrew P. Neilson

    February 24, 2025 08:53 by Researcher Metadata Database

  • Added Creator Joshua D. Lambert

    February 24, 2025 08:53 by Researcher Metadata Database

  • Published

    February 24, 2025 08:53 by Researcher Metadata Database

  • Updated

    February 24, 2025 21:04 by [unknown user]

  • Updated Keyword, Publisher, Description, and 1 more Show Changes

    April 14, 2025 20:33 by avs5190

    Keyword
    • Cocoa, Polyphenols, Dextran sulfate sodium, Inflammation, Ulcerative colitis
    Publisher
    • Die Nahrung
    • Molecular Nutrition and Food Research
    Description
    • Scope: A study is conducted to determine the anti-inflammatory effects of cocoa and polyphenol-rich cocoa fractions in the dextran sulfate sodium (DSS)-induced mouse model of acute colonic inflammation. Methods and results: Male C57BL/6J mice are treated with dietary cocoa powder, an extractable cocoa polyphenol fraction, or a non-extractable cocoa polyphenol fraction for 2 weeks prior to treatment with 2.5% DSS in the drinking water for 7 days to induce colonic inflammation. Cocoa treatment continues during the DSS period. Cocoa and/or cocoa fractions exacerbate DSS-induced weight loss and fail to mitigate DSS-induced colon shortening but do improve splenomegaly. Cocoa/cocoa fraction treatment fails to mitigate DSS-induced mRNA and protein markers of inflammation. Principal component analysis shows overlap between cocoa or cocoa fraction-treated mice and DSS-induced controls, but separation from mice not treated with DSS. Conclusion: The results suggest cocoa and cocoa polyphenols may not be useful in mitigating acute colonic inflammation.
    • Scope: A study is conducted to determine the anti-inflammatory effects of cocoa and polyphenol-rich cocoa fractions in the dextran sulfate sodium (DSS)-induced mouse model of acute colonic inflammation.
    • Methods and results: Male C57BL/6J mice are treated with dietary cocoa powder, an extractable cocoa polyphenol fraction, or a non-extractable cocoa polyphenol fraction for 2 weeks prior to treatment with 2.5% DSS in the drinking water for 7 days to induce colonic inflammation. Cocoa treatment continues during the DSS period. Cocoa and/or cocoa fractions exacerbate DSS-induced weight loss and fail to mitigate DSS-induced colon shortening but do improve splenomegaly. Cocoa/cocoa fraction treatment fails to mitigate DSS-induced mRNA and protein markers of inflammation. Principal component analysis shows overlap between cocoa or cocoa fraction-treated mice and DSS-induced controls, but separation from mice not treated with DSS.
    • Conclusion: The results suggest cocoa and cocoa polyphenols may not be useful in mitigating acute colonic inflammation.
    Publication Date
    • 2024-01-01
    • 2024-07-04