DhFIG_2, a gene of nematode-trapping fungus Dactylellina haptotyla that encodes a component of the low-affinity calcium uptake system, is required for conidiation and knob-trap formation

Calcium ion (Ca2+) is a universal second messenger involved in regulating diverse processes in animals, plants, and fungi. The low-affinity calcium uptake system (LACS) participates in acquiring Ca2+ from extracellular environments under high extracellular Ca2+ concentration. Unlike most fungi, which encode only one protein (FIG1) for LACS, nematode-trapping fungi (NTF) encode two related proteins. AoFIG_2, the NTF-specific LACS component encoded by adhesive network-trap forming Arthrobotrys oligospora, was shown to be required for conidiation and trap formation. We characterized the role of DhFIG_2, an AoFIG_2 ortholog encoded by knob-trap forming Dactylellina haptotyla, in growth and development to expand our understanding of the role of LACS in NTF. Because repeated attempts to disrupt DhFIG_2 failed, knocking down the expression of DhFIG_2 via RNA interference (RNAi) was used to study its function. RNAi of DhFIG_2 significantly decreased its expression, severely reduced conidiation and trap formation, and affected vegetative growth and stress responses, suggesting that this component of LACS is crucial for trap formation and conidiation in NTF. Our study demonstrated the utility of RNAi assisted by ATMT for studying gene function in D. haptotyla.

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Work Title DhFIG_2, a gene of nematode-trapping fungus Dactylellina haptotyla that encodes a component of the low-affinity calcium uptake system, is required for conidiation and knob-trap formation
Access
Open Access
Creators
  1. Xiaozhou Zhao
  2. Yani Fan
  3. Weiwei Zhang
  4. Meichun Xiang
  5. Seogchan Kang
  6. Shunxian Wang
  7. Xingzhong Liu
Keyword
  1. Calcium signaling
  2. Conidiation
  3. Dactylellina haptotyla
  4. Low affinity calcium channel
  5. Nematode trapping
  6. RNA interference
License In Copyright (Rights Reserved)
Work Type Article
Publisher
  1. Fungal Genetics and Biology
Publication Date March 1, 2023
Publisher Identifier (DOI)
  1. https://doi.org/10.1016/j.fgb.2023.103782
Deposited March 13, 2024

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Version 1
published

  • Created
  • Added Zhao__2023_.pdf
  • Added Creator Xiaozhou Zhao
  • Added Creator Yani Fan
  • Added Creator Weiwei Zhang
  • Added Creator Meichun Xiang
  • Added Creator Seogchan Kang
  • Added Creator Shunxian Wang
  • Added Creator Xingzhong Liu
  • Published
  • Updated Description Show Changes
    Description
    • <p>Calcium ion (Ca<sup>2+</sup>) is a universal second messenger involved in regulating diverse processes in animals, plants, and fungi. The low-affinity calcium uptake system (LACS) participates in acquiring Ca<sup>2+</sup> from extracellular environments under high extracellular Ca<sup>2+</sup> concentration. Unlike most fungi, which encode only one protein (FIG1) for LACS, nematode-trapping fungi (NTF) encode two related proteins. AoFIG_2, the NTF-specific LACS component encoded by adhesive network-trap forming Arthrobotrys oligospora, was shown to be required for conidiation and trap formation. We characterized the role of DhFIG_2, an AoFIG_2 ortholog encoded by knob-trap forming Dactylellina haptotyla, in growth and development to expand our understanding of the role of LACS in NTF. Because repeated attempts to disrupt DhFIG_2 failed, knocking down the expression of DhFIG_2 via RNA interference (RNAi) was used to study its function. RNAi of DhFIG_2 significantly decreased its expression, severely reduced conidiation and trap formation, and affected vegetative growth and stress responses, suggesting that this component of LACS is crucial for trap formation and conidiation in NTF. Our study demonstrated the utility of RNAi assisted by ATMT for studying gene function in D. haptotyla.</p>
    • <p>Calcium ion (Ca<sup>2+</sup>) is a universal second messenger involved in regulating diverse processes in animals, plants, and fungi. The low-affinity calcium uptake system (LACS) participates in acquiring Ca<sup>2+</sup> from extracellular environments under high extracellular Ca<sup>2+</sup> concentration. Unlike most fungi, which encode only one protein (FIG1) for LACS, nematode-trapping fungi (NTF) encode two related proteins. \AoFIG_2\, the NTF-specific LACS component encoded by adhesive network-trap forming _Arthrobotrys oligospora_, was shown to be required for conidiation and trap formation. We characterized the role of \DhFIG_2, an AoFIG_2\ ortholog encoded by knob-trap forming _Dactylellina haptotyla_, in growth and development to expand our understanding of the role of LACS in NTF. Because repeated attempts to disrupt \DhFIG_2\ failed, knocking down the expression of \DhFIG_2\ via RNA interference (RNAi) was used to study its function. RNAi of \DhFIG_2\ significantly decreased its expression, severely reduced conidiation and trap formation, and affected vegetative growth and stress responses, suggesting that this component of LACS is crucial for trap formation and conidiation in NTF. Our study demonstrated the utility of RNAi assisted by ATMT for studying gene function in _D. haptotyla_.</p>
  • Updated Description Show Changes
    Description
    • <p>Calcium ion (Ca<sup>2+</sup>) is a universal second messenger involved in regulating diverse processes in animals, plants, and fungi. The low-affinity calcium uptake system (LACS) participates in acquiring Ca<sup>2+</sup> from extracellular environments under high extracellular Ca<sup>2+</sup> concentration. Unlike most fungi, which encode only one protein (FIG1) for LACS, nematode-trapping fungi (NTF) encode two related proteins. \AoFIG_2\, the NTF-specific LACS component encoded by adhesive network-trap forming _Arthrobotrys oligospora_, was shown to be required for conidiation and trap formation. We characterized the role of \DhFIG_2, an AoFIG_2\ ortholog encoded by knob-trap forming _Dactylellina haptotyla_, in growth and development to expand our understanding of the role of LACS in NTF. Because repeated attempts to disrupt \DhFIG_2\ failed, knocking down the expression of \DhFIG_2\ via RNA interference (RNAi) was used to study its function. RNAi of \DhFIG_2\ significantly decreased its expression, severely reduced conidiation and trap formation, and affected vegetative growth and stress responses, suggesting that this component of LACS is crucial for trap formation and conidiation in NTF. Our study demonstrated the utility of RNAi assisted by ATMT for studying gene function in _D. haptotyla_.</p>
    • <p>Calcium ion (Ca<sup>2+</sup>) is a universal second messenger involved in regulating diverse processes in animals, plants, and fungi. The low-affinity calcium uptake system (LACS) participates in acquiring Ca<sup>2+</sup> from extracellular environments under high extracellular Ca<sup>2+</sup> concentration. Unlike most fungi, which encode only one protein (FIG1) for LACS, nematode-trapping fungi (NTF) encode two related proteins. AoFIG\_2, the NTF-specific LACS component encoded by adhesive network-trap forming _Arthrobotrys oligospora_, was shown to be required for conidiation and trap formation. We characterized the role of DhFIG\_2, an AoFIG\_2 ortholog encoded by knob-trap forming _Dactylellina haptotyla_, in growth and development to expand our understanding of the role of LACS in NTF. Because repeated attempts to disrupt DhFIG\_2 failed, knocking down the expression of DhFIG\_2 via RNA interference (RNAi) was used to study its function. RNAi of DhFIG\_2 significantly decreased its expression, severely reduced conidiation and trap formation, and affected vegetative growth and stress responses, suggesting that this component of LACS is crucial for trap formation and conidiation in NTF. Our study demonstrated the utility of RNAi assisted by ATMT for studying gene function in _D. haptotyla_.</p>
  • Updated Keyword, Publication Date Show Changes
    Keyword
    • Calcium signaling, Conidiation, Dactylellina haptotyla, Low affinity calcium channel, Nematode trapping, RNA interference
    Publication Date
    • 2023-05-01
    • 2023-03-01
  • Updated