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Created
March 13, 2024 22:28
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Researcher Metadata Database
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Added
Zhao__2023_.pdf
March 13, 2024 22:28
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Researcher Metadata Database
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Added Creator Xiaozhou Zhao
March 13, 2024 22:28
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Researcher Metadata Database
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Added Creator Yani Fan
March 13, 2024 22:28
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Researcher Metadata Database
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Added Creator Weiwei Zhang
March 13, 2024 22:28
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Researcher Metadata Database
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Added Creator Meichun Xiang
March 13, 2024 22:28
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Researcher Metadata Database
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Added Creator Seogchan Kang
March 13, 2024 22:28
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Researcher Metadata Database
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Added Creator Shunxian Wang
March 13, 2024 22:28
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Researcher Metadata Database
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Added Creator Xingzhong Liu
March 13, 2024 22:28
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Researcher Metadata Database
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Published
March 13, 2024 22:28
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Researcher Metadata Database
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March 15, 2024 12:56
by
avs5190
Description
<p>Calcium ion (Ca<sup>2+</sup>) is a universal second messenger involved in regulating diverse processes in animals, plants, and fungi. The low-affinity calcium uptake system (LACS) participates in acquiring Ca<sup>2+</sup> from extracellular environments under high extracellular Ca<sup>2+</sup> concentration. Unlike most fungi, which encode only one protein (FIG1) for LACS, nematode-trapping fungi (NTF) encode two related proteins. AoFIG_2, the NTF-specific LACS component encoded by adhesive network-trap forming Arthrobotrys oligospora, was shown to be required for conidiation and trap formation. We characterized the role of DhFIG_2, an AoFIG_2 ortholog encoded by knob-trap forming Dactylellina haptotyla, in growth and development to expand our understanding of the role of LACS in NTF. Because repeated attempts to disrupt DhFIG_2 failed, knocking down the expression of DhFIG_2 via RNA interference (RNAi) was used to study its function. RNAi of DhFIG_2 significantly decreased its expression, severely reduced conidiation and trap formation, and affected vegetative growth and stress responses, suggesting that this component of LACS is crucial for trap formation and conidiation in NTF. Our study demonstrated the utility of RNAi assisted by ATMT for studying gene function in D. haptotyla.</p>
- <p>Calcium ion (Ca<sup>2+</sup>) is a universal second messenger involved in regulating diverse processes in animals, plants, and fungi. The low-affinity calcium uptake system (LACS) participates in acquiring Ca<sup>2+</sup> from extracellular environments under high extracellular Ca<sup>2+</sup> concentration. Unlike most fungi, which encode only one protein (FIG1) for LACS, nematode-trapping fungi (NTF) encode two related proteins. \AoFIG_2\, the NTF-specific LACS component encoded by adhesive network-trap forming _Arthrobotrys oligospora_, was shown to be required for conidiation and trap formation. We characterized the role of \DhFIG_2, an AoFIG_2\ ortholog encoded by knob-trap forming _Dactylellina haptotyla_, in growth and development to expand our understanding of the role of LACS in NTF. Because repeated attempts to disrupt \DhFIG_2\ failed, knocking down the expression of \DhFIG_2\ via RNA interference (RNAi) was used to study its function. RNAi of \DhFIG_2\ significantly decreased its expression, severely reduced conidiation and trap formation, and affected vegetative growth and stress responses, suggesting that this component of LACS is crucial for trap formation and conidiation in NTF. Our study demonstrated the utility of RNAi assisted by ATMT for studying gene function in _D. haptotyla_.</p>
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March 15, 2024 12:57
by
avs5190
Description
<p>Calcium ion (Ca<sup>2+</sup>) is a universal second messenger involved in regulating diverse processes in animals, plants, and fungi. The low-affinity calcium uptake system (LACS) participates in acquiring Ca<sup>2+</sup> from extracellular environments under high extracellular Ca<sup>2+</sup> concentration. Unlike most fungi, which encode only one protein (FIG1) for LACS, nematode-trapping fungi (NTF) encode two related proteins. \AoFIG_2\, the NTF-specific LACS component encoded by adhesive network-trap forming _Arthrobotrys oligospora_, was shown to be required for conidiation and trap formation. We characterized the role of \DhFIG_2, an AoFIG_2\ ortholog encoded by knob-trap forming _Dactylellina haptotyla_, in growth and development to expand our understanding of the role of LACS in NTF. Because repeated attempts to disrupt \DhFIG_2\ failed, knocking down the expression of \DhFIG_2\ via RNA interference (RNAi) was used to study its function. RNAi of \DhFIG_2\ significantly decreased its expression, severely reduced conidiation and trap formation, and affected vegetative growth and stress responses, suggesting that this component of LACS is crucial for trap formation and conidiation in NTF. Our study demonstrated the utility of RNAi assisted by ATMT for studying gene function in _D. haptotyla_.</p>
- <p>Calcium ion (Ca<sup>2+</sup>) is a universal second messenger involved in regulating diverse processes in animals, plants, and fungi. The low-affinity calcium uptake system (LACS) participates in acquiring Ca<sup>2+</sup> from extracellular environments under high extracellular Ca<sup>2+</sup> concentration. Unlike most fungi, which encode only one protein (FIG1) for LACS, nematode-trapping fungi (NTF) encode two related proteins. AoFIG\_2, the NTF-specific LACS component encoded by adhesive network-trap forming _Arthrobotrys oligospora_, was shown to be required for conidiation and trap formation. We characterized the role of DhFIG\_2, an AoFIG\_2 ortholog encoded by knob-trap forming _Dactylellina haptotyla_, in growth and development to expand our understanding of the role of LACS in NTF. Because repeated attempts to disrupt DhFIG\_2 failed, knocking down the expression of DhFIG\_2 via RNA interference (RNAi) was used to study its function. RNAi of DhFIG\_2 significantly decreased its expression, severely reduced conidiation and trap formation, and affected vegetative growth and stress responses, suggesting that this component of LACS is crucial for trap formation and conidiation in NTF. Our study demonstrated the utility of RNAi assisted by ATMT for studying gene function in _D. haptotyla_.</p>
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March 15, 2024 12:58
by
avs5190
Keyword
- Calcium signaling, Conidiation, Dactylellina haptotyla, Low affinity calcium channel, Nematode trapping, RNA interference
Publication Date
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Updated
April 04, 2024 10:22
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[unknown user]