Surface Plasmon Resonance Analysis for Quantifying Protein-Carbohydrate Interactions

During the past two decades, surface plasmon resonance (SPR) analysis has emerged as an important tool for studying protein-carbohydrate interactions, with several commercial instruments available. Binding affinities in the nM to mM range can be determined; however, there are pitfalls that require careful experimental design to avoid. Here we give an overview of each step in the SPR analysis from immobilization to data analysis, providing key points of consideration that will allow practitioners to achieve reliable and reproducible results.

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Work Title Surface Plasmon Resonance Analysis for Quantifying Protein-Carbohydrate Interactions
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Open Access
Creators
  1. Marie Sofie Møller
  2. Darrell W. Cockburn
  3. Casper Wilkens
Keyword
  1. Enzyme
  2. Protein-carbohydrate interactions
  3. Surface plasmon resonance
  4. Carbohydrate-binding module
  5. Carbohydrate-active enzyme
  6. Biosensor
License In Copyright (Rights Reserved)
Work Type Article
Publisher
  1. Carbohydrate-Protein Interactions
Publication Date May 7, 2023
Publisher Identifier (DOI)
  1. https://doi.org/10.1007/978-1-0716-3151-5_10
Deposited March 21, 2024

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Version 1
published

  • Created
  • Added Moller_2023_Methods_in_Molecular_Biology.pdf
  • Added Creator Marie Sofie Møller
  • Added Creator Darrell W. Cockburn
  • Added Creator Casper Wilkens
  • Published
  • Updated Keyword, Publisher Show Changes
    Keyword
    • Enzyme, Protein-carbohydrate interactions, Surface plasmon resonance, Carbohydrate-binding module, Carbohydrate-active enzyme, Biosensor
    Publisher
    • Methods in Molecular Biology
    • Carbohydrate-Protein Interactions
  • Updated