Assessing the off-target movement of tebufenozide in forested ecosystems: Implications for vernal pond ecosystems

The widespread use of pesticides has significantly contributed to managing pest populations in both agricultural and forest ecosystems yet concerns about their unintended impacts on non-target habitats continue to grow. Tebufenozide, a pesticide commonly used to control forest defoliator pests, including spongy moth (Lymantria dispar dispar), is known for its selective action on Lepidopteran larvae. Despite its targeted mode of action, the potential transport and fate of tebufenozide into sensitive forested aquatic habitats, such as vernal ponds, is not well understood. This study examines the spatial distribution of tebufenozide in 41 vernal ponds located within three state forests in central Pennsylvania (Bald Eagle, Rothrock, and Tuscarora) by analyzing sediment and water samples collected within and outside designated spray blocks. Tebufenozide was detected in 39 water samples and 40 sediment samples, including 27 unsprayed water and sediment samples, indicating possible pesticide drift or runoff into non-target areas. We used a Mann-Whitney U test to reveal significantly higher concentrations of tebufenozide in ponds within spray blocks for both sediment (W = 241.5, p = 0.0161) and water (W = 316.5, p = 2.769e-06). Tebufenozide concentrations were higher in ponds closer to spray zones, suggesting proximity influences pesticide levels, though no clear directional dispersal patterns emerged. These findings underscore the vulnerability of vernal ponds, essential breeding habitats for amphibians and other organisms, to pesticide contamination. Enhanced management strategies, such as wider buffer zones and alternative pest control measures, may be necessary to safeguard these critical ecosystems.

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Ward, Mason; Sweetman, Jon (2025). Assessing the off-target movement of tebufenozide in forested ecosystems: Implications for vernal pond ecosystems [Data set]. Scholarsphere. https://doi.org/10.26207/g55c-bj36

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Work Title Assessing the off-target movement of tebufenozide in forested ecosystems: Implications for vernal pond ecosystems
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Open Access
Creators
  1. Mason Ward
  2. Jon Sweetman
License MIT License
Work Type Dataset
Acknowledgments
  1. We thank undergraduate technicians E. Roush, J. Smith, and K. Lentzsch for critical contributions to fieldwork and processing laboratory samples. We also thank the EESL at The Pennsylvania State University for pesticide analysis. MSW is supported by the National Science Foundation Graduate Research Fellowship Program under Grant No. DGE1255832. JNS is supported by the USDA National Institute of Food and Agriculture under Project #PEN04819 and Accession #7003691. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.
Publication Date 2025
DOI doi:10.26207/g55c-bj36
Deposited June 27, 2025

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  • Created
  • Updated
  • Updated Description, Publication Date Show Changes
    Description
    • The widespread use of pesticides has significantly contributed to managing pest populations in both agricultural and forest ecosystems yet concerns about their unintended impacts on non-target habitats continue to grow. Tebufenozide, a pesticide commonly used to control forest defoliator pests, including spongy moth (Lymantria dispar dispar), is known for its selective action on Lepidopteran larvae. Despite its targeted mode of action, the potential transport and fate of tebufenozide into sensitive forested aquatic habitats, such as vernal ponds, is not well understood. This study examines the spatial distribution of tebufenozide in 41 vernal ponds located within three state forests in central Pennsylvania (Bald Eagle, Rothrock, and Tuscarora) by analyzing sediment and water samples collected within and outside designated spray blocks. Tebufenozide was detected in 39 water samples and 40 sediment samples, including 27 unsprayed water and sediment samples, indicating possible pesticide drift or runoff into non-target areas. We used a Mann-Whitney U test to reveal significantly higher concentrations of tebufenozide in ponds within spray blocks for both sediment (W = 241.5, p = 0.0161) and water (W = 316.5, p = 2.769e-06). Tebufenozide concentrations were higher in ponds closer to spray zones, suggesting proximity influences pesticide levels, though no clear directional dispersal patterns emerged. These findings underscore the vulnerability of vernal ponds, essential breeding habitats for amphibians and other organisms, to pesticide contamination. Enhanced management strategies, such as wider buffer zones and alternative pest control measures, may be necessary to safeguard these critical ecosystems.
    Publication Date
    • 2025
  • Updated Acknowledgments Show Changes
    Acknowledgments
    • We thank undergraduate technicians E. Roush, J. Smith, and K. Lentzsch for critical contributions to fieldwork and processing laboratory samples. We also thank the EESL at The Pennsylvania State University for pesticide analysis. MSW is supported by the National Science Foundation Graduate Research Fellowship Program under Grant No. DGE1255832. JNS is supported by the USDA National Institute of Food and Agriculture under Project #PEN04819 and Accession #7003691. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.
  • Added Creator Mason Ward
  • Added Creator Jon Sweetman
  • Added teb_spray_data.csv
  • Updated License Show Changes
    License
    • https://opensource.org/licenses/MIT
  • Added WardSweetman_TebSpatial_README.txt
  • Published
  • Updated