High-Resolution Imaging of Human Viruses in Liquid Droplets

Liquid-phase electron microscopy (LP-EM) is an exciting new area in the materials imaging field, providing unprecedented views of molecular processes. Time-resolved insights from LP-EM studies are a strong complement to the remarkable results achievable with other high-resolution techniques. Here, the opportunities to expand LP-EM technology beyond 2D temporal assessments and into the 3D regime are described. The results show new structures and dynamic insights of human viruses contained in minute volumes of liquid while acquired in a rapid timeframe. To develop this strategy, adeno-associated virus (AAV) is used as a model system. AAV is a well-known gene therapy vehicle with current applications involving drug delivery and vaccine development for COVID-19. Improving the understanding of the physical properties of biological entities in a liquid state, as maintained in the human body, has broad societal implications for human health and disease.



Work Title High-Resolution Imaging of Human Viruses in Liquid Droplets
Open Access
  1. G. M. Jonaid
  2. William J. Dearnaley
  3. Michael A. Casasanta
  4. Liam Kaylor
  5. Samantha Berry
  6. Madeline J. Dukes
  7. Michael S. Spilman
  8. Jennifer L. Gray
  9. Deborah F. Kelly
License In Copyright (Rights Reserved)
Work Type Article
  1. Advanced Materials
Publication Date September 16, 2021
Publisher Identifier (DOI)
  1. https://doi.org/10.1002/adma.202103221
Deposited July 19, 2022




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Work History

Version 1

  • Created
  • Added Advanced_Materials_final.pdf
  • Added Creator G. M. Jonaid
  • Added Creator William J. Dearnaley
  • Added Creator Michael A. Casasanta
  • Added Creator Liam Kaylor
  • Added Creator Samantha Berry
  • Added Creator Madeline J. Dukes
  • Added Creator Michael S. Spilman
  • Added Creator Jennifer L. Gray
  • Added Creator Deborah F. Kelly
  • Published