HCMV infection downregulates GPX4 and stimulates lipid peroxidation but does not induce ferroptosis Figure 8

Ferroptosis is differentially regulated during HCMV infection. (A) Lipid peroxidation and total lipid measured with C11 BODIPY FITC (green) and phycoerythrin (PE) (red) fluorescence in MRC-5 cells either uninfected (0 hpi) or TB40/E-WT (MOI = 3) infected at 24, 48, 72, 96, and 120 hpi. Fluorescence is shown relative to uninfected cells. Results are from two individual experiments, n = 6. (B) LDH release measured in uninfected MRC-5 cells or infected MRC-5 cells (as described in panel A) at 48 hpi following 24 hour treatment with RSL3 at twofold serial dilutions from 2–0.015625 μM. Data are shown as the percentage of LDH release relative to the maximum LDH levels with the equation (experimental LDH – spontaneous LDH)/(maximum LDH – spontaneous LDH). Results are from two independent experiments, n = 6. (C) LDH release measured in uninfected MRC-5 cells infected MRC-5 cells (as described in panel A) at 72 hpi following 24-hour treatment with RSL3 at twofold serial dilutions from 2–0.015625 μM. Data are shown as described in panel B. Results are from two independent experiments, n = 6. (D) LDH release measured in uninfected MRC-5 cells or infected MRC-5 cells (as described in panel A) at 96 hpi following 24-hour treatment with RSL3 at twofold serial dilutions from 2–0.015625 μM. Data are shown as described in panel B. Results are from four independent experiments, n = 12. For the above panels, * indicates P < 0.05 and ns = not significant.

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Norbury, Christopher (2024). HCMV infection downregulates GPX4 and stimulates lipid peroxidation but does not induce ferroptosis Figure 8 [Data set]. Scholarsphere.

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Work Title HCMV infection downregulates GPX4 and stimulates lipid peroxidation but does not induce ferroptosis Figure 8
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Open Access
Creators
  1. Christopher Norbury
License CC BY 4.0 (Attribution)
Work Type Dataset
Publication Date December 20, 2024
Deposited December 20, 2024

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  • Updated Description, Publication Date Show Changes
    Description
    • Ferroptosis is differentially regulated during HCMV infection. (A) Lipid peroxidation and total lipid measured with C11 BODIPY FITC (green) and phycoerythrin (PE) (red) fluorescence in MRC-5 cells either uninfected (0 hpi) or TB40/E-WT (MOI = 3) infected at 24, 48, 72, 96, and 120 hpi. Fluorescence is shown relative to uninfected cells. Results are from two individual experiments, n = 6. (B) LDH release measured in uninfected MRC-5 cells or infected MRC-5 cells (as described in panel A) at 48 hpi following 24 hour treatment with RSL3 at twofold serial dilutions from 2–0.015625 μM. Data are shown as the percentage of LDH release relative to the maximum LDH levels with the equation (experimental LDH – spontaneous LDH)/(maximum LDH – spontaneous LDH). Results are from two independent experiments, n = 6. (C) LDH release measured in uninfected MRC-5 cells infected MRC-5 cells (as described in panel A) at 72 hpi following 24-hour treatment with RSL3 at twofold serial dilutions from 2–0.015625 μM. Data are shown as described in panel B. Results are from two independent experiments, n = 6. (D) LDH release measured in uninfected MRC-5 cells or infected MRC-5 cells (as described in panel A) at 96 hpi following 24-hour treatment with RSL3 at twofold serial dilutions from 2–0.015625 μM. Data are shown as described in panel B. Results are from four independent experiments, n = 12. For the above panels, * indicates P < 0.05 and ns = not significant.
    Publication Date
    • 2024-12-20
  • Added 012924 C11 BODIPY timecourse analysis redo.xlsx
  • Updated
  • Added 011624 C11 BODIPY timecourse.wsp
  • Added Compensation Controls_FITC Stained Control.fcs
  • Added Compensation Controls_PE Stained Control.fcs
  • Added Compensation Controls_Unstained Control.fcs
  • Added Specimen_001_24hpi 1.fcs
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  • Added Specimen_001_US.fcs
  • Added Creator Christopher Norbury
  • Added Figure 8.pdf
  • Added Fig8 readme.txt
  • Updated License Show Changes
    License
    • https://creativecommons.org/licenses/by/4.0/
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