Effect of Membrane Pore Structure on Fouling Behavior of Glycoconjugate Vaccines

Sterile filtration can be difficult when processing large glycoconjugate vaccines. The objective of this work was to evaluate the performance of three commercial 0.2 µm nominal pore size sterilizing grade membranes with very different morphology: Millipore Durapore® (homogeneous), Millipore Express® (asymmetric), and Sartopore® Platinum (dual layer) during sterile filtration of a glycoconjugate vaccine provided by Pfizer. The location of deposited glycoconjugate within the filters was examined by confocal microscopy. The greatest capacity was obtained with the asymmetric membrane due to its high permeability, while the lowest capacity was found with the two-layer membrane in which the fouling occurred at the entrance to the 0.2 µm layer. The pressure profiles during constant flux filtration were analyzed using a modified intermediate pore blockage model accounting for the fouled and unfouled regions within the filter, providing additional insights into the effects of membrane morphology on the fouling characteristics.

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Work Title Effect of Membrane Pore Structure on Fouling Behavior of Glycoconjugate Vaccines
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Open Access
Creators
  1. Andrew Zydney
  2. Parinaz Emami
  3. Fatemeh Fallahianbijan
  4. Jordan M Hillsley
  5. Seyed Pouria Motevalian
  6. Brenda Carrillo Conde
  7. Kelvin Reilly
Keyword
  1. Sterile Filtration, Vaccines, Membrane Morphology, Glycoconjugates, Fouling
License CC BY-NC-ND 4.0 (Attribution-NonCommercial-NoDerivatives)
Work Type Article
Publisher
  1. Elsevier
Publication Date October 2, 2020
Publisher Identifier (DOI)
  1. 10.1016/j.memsci.2020.118797
Deposited March 07, 2021

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Version 1
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  • Created
  • Added Creator Andrew Zydney
  • Added Creator Parinaz Emami
  • Added Creator Fatemeh Fallahianbijan
  • Added Creator Jordan M Hillsley
  • Added Creator Seyed Pouria Motevalian
  • Added Creator Brenda Carrillo Conde
  • Added Creator Kelvin Reilly
  • Added Glycoconjugate-sterile filter comparison-revised.docx
  • Updated License Show Changes
    License
    • https://creativecommons.org/licenses/by-nc-nd/4.0/
  • Published
  • Updated