HCMV infection downregulates GPX4 and stimulates lipid peroxidation but does not induce ferroptosis Figure S1

RSL3 off-target effect on TXNRD does not affect viral replication. (A) Infectious titers at 120 hpi from TB40/E-WT (MOI=3) infected MRC-5 cells treated with vehicle control (DMSO), RSL3 (1M), or Tri-1 (1M) at 72 hpi. Results are from 2 independent experiments, n=6. (B) TXNRD activity (in nmol/min/mL) in MRC-5 cells infected with TB40/E-WT (MOI=3) and treated with DMSO, RSL3 (1M), or Tri-1 (1M) at 72 hpi and harvested at 120hpi. Results are from 1 independent experiment, n=3. For the above panels, * indicates p<0.05, and ns = not significant.

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Norbury, Christopher (2024). HCMV infection downregulates GPX4 and stimulates lipid peroxidation but does not induce ferroptosis Figure S1 [Data set]. Scholarsphere.

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Work Title HCMV infection downregulates GPX4 and stimulates lipid peroxidation but does not induce ferroptosis Figure S1
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Open Access
Creators
  1. Christopher Norbury
License CC BY 4.0 (Attribution)
Work Type Dataset
Publication Date December 20, 2024
Deposited December 20, 2024

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Version 1
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  • Updated Description, Publication Date Show Changes
    Description
    • RSL3 off-target effect on TXNRD does not affect viral replication. (A) Infectious titers at 120 hpi from TB40/E-WT (MOI=3) infected MRC-5 cells treated with vehicle control (DMSO), RSL3 (1M), or Tri-1 (1M) at 72 hpi. Results are from 2 independent experiments, n=6. (B) TXNRD activity (in nmol/min/mL) in MRC-5 cells infected with TB40/E-WT (MOI=3) and treated with DMSO, RSL3 (1M), or Tri-1 (1M) at 72 hpi and harvested at 120hpi. Results are from 1 independent experiment, n=3. For the above panels, * indicates p<0.05, and ns = not significant.
    Publication Date
    • 2024-12-20
  • Added 090524 TXNRD inhibitor titers combined.xlsx
  • Added 091124 TXNRD assay data and analysis.xlsx
  • Added 090424 TXNRD inhibitors set 2.xlsx
  • Added 090424 TXNRD inhibitors.xlsx
  • Added 091124 TXNRD assay protein calculations.xlsx
  • Added Creator Christopher Norbury
  • Added Supplemental Figure CN 122224.docx
  • Deleted Supplemental Figure CN 122224.docx
  • Added Supplemental Figure CN 122224.pdf
  • Added FigS1 readme.txt
  • Updated License Show Changes
    License
    • https://creativecommons.org/licenses/by/4.0/
  • Published
  • Updated