The role of toxic nectar secondary compounds in driving differential bumble bee preferences for milkweed flowers

While morphological differences such as tongue length are often featured drivers of pollinator floral preferences, differences in chemical detection and tolerance to secondary compounds may also play a role. We sought to better understand the role of secondary compounds in floral preference by examining visitation of milkweed flowers, which can contain toxic, cardenolides in their nectar, by bumble bees (Bombus spp.), some of their most abundant and important pollinators. We examine bumble bee species visitation of common milkweed (Asclepias syriaca) compared to other flowers in the field, and test whether observed preferences may be influenced by avoidance and tolerance of cardenolides, as measured by the cardenolide, ouabain, in the lab. We reveal that common milkweed is vitiated predominantly by one bumble bee species, Bombus griseocollis, in a ratio much higher than the abundance of this species in the community. We confirmed the presence and toxicity of cardenolides in A. syriaca nectar. Lab experiments revealed that B. griseocollis, compared to common bumble bees, B. impatiens, and B. bimaculatus, exhibit greater avoidance of cardenolides, but only at levels that start to induce illness, whereas the other species exhibit either no or reduced avoidance of cardenolides, resulting in illness and mortality in these bees. Toxicity experiments reveal that B. griseocollis also has a substantially higher tolerance for cardenolides than B. impatiens. Together these results support a potential evolutionary association between B. griseocollis and milkweed that may involve increased ability to both detect and tolerate milkweed cardenolides. More information can be found in the manuscript: Villalona E, Ezray B.D., Laveaga E, Anurag A.A., Ali J.G., and Hines H.M. (2020) The role of toxic nectar secondary compounds in driving differential bumble bee preferences for milkweed flowers. Oecologia. Here, we share the associated data including raw data from field visitation observations, preference trials, toxicity trials, and HPLC data along with relevant scripts for analysis.

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Work Title The role of toxic nectar secondary compounds in driving differential bumble bee preferences for milkweed flowers
Access
Open Access
Creators
  1. Eris Villalona
  2. Briana D. Ezray
  3. Erica Laveaga
  4. Anurag A. Agrawal
  5. Jared G. Ali
  6. Heather M. Hines
Keyword
  1. niche partitioning
  2. cardiac glycosides
  3. Asclepias
  4. Bombus
  5. plant-pollinator interactions
  6. detoxification
  7. specialization
License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
Work Type Software Or Program Code
Acknowledgments
  1. Swayamijit Ray
  2. Alex Serpi
  3. Ashley Heimann
Publisher
  1. Oecologia
Publication Date 2020
DOI doi:10.26207/pgeq-he51
Deposited June 18, 2020

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Version 1
published

  • Created
  • Added Raw_Data_Files_FieldPreferenceToxicity.xlsx
  • Added OECO-D-20-00056R1_AssociatedData_Archival.zip
  • Added iNaturalistRecords.xlsx
  • Added Creator Eris Villalona
  • Added Creator Briana D. Ezray
  • Added Creator Erica Laveaga
  • Added Creator Anurag A. Agrawal
  • Added Creator Jared G. Ali
  • Added Creator Heather M. Hines
  • Published