Dissolution and In vitro Wound Healing Performance of Chemically Aided Milled Bioactive Glass

In this study, bioactive glass compositions 45S5 and 13-93B3 were processed using attrition milling to produce nanoparticles. Static dissolution and in vitro testing were employed to determine the effect of size and composition on release of therapeutic ions, cell growth, migration. Attrition milling produced a mean particles size below 300 nanometers for both compositions after 2 hours of milling. Static dissolution determined that boron has a lower activation barrier for dissolution than silicon. Cell proliferation using an MTS assay shows that bioactive glass treatments do not stimulate proliferation but can cause cell death if pH rises above the healthy range. A in vitro scratch test was applied to determine the effect of composition and particle size on cell migration. Both 45S5 and 13-93B3 show increased migration rates over no treatment. After 24 hours, 13-93B3 induces 60% wound closure, 45S5 shows 45% and no treatment shows 26%.

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Work Title Dissolution and In vitro Wound Healing Performance of Chemically Aided Milled Bioactive Glass
Access
Open Access
Creators
  1. Adam Shearer
  2. Brett Rosoff-Verbit
  3. Bernadette Adair
  4. James H. Adair
  5. John C. Mauro
Keyword
  1. Bioglass
  2. Glass dissolution
  3. Nanoparticles
  4. Wound healing
License No Copyright - U.S.
Work Type Research Paper
Publication Date July 2022
Deposited October 10, 2023

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Version 1
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  • Created
  • Updated
  • Updated Acknowledgments Show Changes
    Acknowledgments
    • Brett Rosoff-Verbit, Bernadette Adair, James H. Adair, John C. Mauro
  • Added Creator Adam Shearer
  • Updated License Show Changes
    License
    • https://rightsstatements.org/page/NoC-US/1.0/
  • Added Shearer-Adam_2022.pdf
  • Published
  • Updated Keyword Show Changes
    Keyword
    • bioglass, glass dissolution, nanoparticles, wound healing
    • Bioglass, Glass dissolution, Nanoparticles, Wound healing
  • Updated Acknowledgments Show Changes
    Acknowledgments
    • Brett Rosoff-Verbit, Bernadette Adair, James H. Adair, John C. Mauro
  • Added Creator Brett Rosoff-Verbit
  • Added Creator Bernadette Adair
  • Added Creator James H. Adair
  • Added Creator John C. Mauro
  • Updated