Data for "Surgical Debulking Modifies Notch Signaling and May Improve Vismodegib Effectiveness for Locally Advanced Basal Cell Carcinoma"

Smoothened (SMO) inhibitors, such as vismodegib, exhibit remarkable success in treating patients with locally advanced basal cell carcinoma (LaBCC). Yet, vismodegib efficacy is hindered by notable side effects, which often lead to treatment discontinuation and subsequent relapse in LaBCC patients. Prolonged remission was previously reported in patients with LaBCCs who underwent surgical debulking before starting vismodegib. Here, we enrolled four patients with LaBCC who underwent debulking followed by vismodegib therapy to assess their clinical outcomes and analyze the cutaneous molecular changes occurring as a result of surgical intervention. Following LaBCC debulking, patients underwent a punch biopsy of residual BCC tissue one week later. RT-qPCR analysis of 24 Notch and Wnt signaling-associated genes revealed elevated PTCH1, HEY2, LGR6, FZD2, LEF1, ALCAM, and RUNX1 expression in follow-up biopsies compared to patient-matched debulked tissue. Immunoblot and immunostaining further confirmed elevated Notch signaling in follow-up biopsy tissue compared to patient-matched debulked tumor tissue. Patients 1, 3, and 4 displayed a clinical response to debulking followed by vismodegib, while Patient 2 was lost to follow-up post-debulk. These findings suggest that surgical manipulation of LaBCCs is correlated with molecular alterations in signaling pathways associated with cellular reprogramming.

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Hobbs, Ryan; Lam, Charlene (2024). Data for "Surgical Debulking Modifies Notch Signaling and May Improve Vismodegib Effectiveness for Locally Advanced Basal Cell Carcinoma" [Data set]. Scholarsphere.

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Work Title Data for "Surgical Debulking Modifies Notch Signaling and May Improve Vismodegib Effectiveness for Locally Advanced Basal Cell Carcinoma"
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Open Access
Creators
  1. Ryan Hobbs
  2. Charlene Lam
Keyword
  1. Basal cell carcinoma
  2. Notch signaling
  3. Smoothened inhibitors
License CC BY 4.0 (Attribution)
Work Type Dataset
Publication Date June 5, 2024
Related URLs
Deposited June 25, 2024

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Version 1
published

  • Created
  • Updated
  • Updated
  • Updated Description, Publication Date Show Changes
    Description
    • Smoothened (SMO) inhibitors, such as vismodegib, exhibit remarkable success in treating patients with locally advanced basal cell carcinoma (LaBCC). Yet, vismodegib efficacy is hindered by notable side effects, which often lead to treatment discontinuation and subsequent relapse in LaBCC patients. Prolonged remission was previously reported in patients with LaBCCs who underwent surgical debulking before starting vismodegib. Here, we enrolled four patients with LaBCC who underwent debulking followed by vismodegib therapy to assess their clinical outcomes and analyze the cutaneous molecular changes occurring as a result of surgical intervention. Following LaBCC debulking, patients underwent a punch biopsy of residual BCC tissue one week later. RT-qPCR analysis of 24 Notch and Wnt signaling-associated genes revealed elevated PTCH1, HEY2, LGR6, FZD2, LEF1, ALCAM, and RUNX1 expression in follow-up biopsies compared to patient-matched debulked tissue. Immunoblot and immunostaining further confirmed elevated Notch signaling in follow-up biopsy tissue compared to patient-matched debulked tumor tissue. Patients 1, 3, and 4 displayed a clinical response to debulking followed by vismodegib, while Patient 2 was lost to follow-up post-debulk. These findings suggest that surgical manipulation of LaBCCs is correlated with molecular alterations in signaling pathways associated with cellular reprogramming.
    Publication Date
    • 2024-06-05
  • Added Creator Ryan Hobbs
  • Added Creator Charlene Lam
  • Added BCC debulk_IF analysis_10.28.22.xlsx
  • Added BCC debulk_protein quantification.xlsx
  • Added BCC debulk_qPCR raw Cq_6.3.22.xlsx
  • Updated License Show Changes
    License
    • https://creativecommons.org/licenses/by/4.0/
  • Published
  • Updated
  • Updated Work Title Show Changes
    Work Title
    • Surgical debulking modifies Notch signaling and may improve vismodegib effectiveness for locally advanced basal cell carcinoma
    • Data for "Surgical Debulking Modifies Notch Signaling and May Improve Vismodegib Effectiveness for Locally Advanced Basal Cell Carcinoma"
  • Updated Keyword, Related URLs Show Changes
    Keyword
    • Basal cell carcinoma, Notch signaling, Smoothened inhibitors
    Related URLs
    • https://doi.org/10.1016/j.xjidi.2024.100288

Version 2
published

  • Created
  • Added readme.txt
  • Updated Subtitle, Publisher Show Changes
    Subtitle
    • Journal of Investigative Dermatology Innovations
    Publisher
    • Elsevier
  • Updated
  • Published
  • Updated
  • Updated Subtitle, Publisher Show Changes
    Subtitle
    • Journal of Investigative Dermatology Innovations
    Publisher
    • Elsevier