Analysis of tradeoffs between purification factor and yield for high-performance countercurrent membrane purification for protein separations
High-performance countercurrent membrane purification (HPCMP) has recently been presented as a new approach for protein separations, exploiting differences in diffusive transport across a semipermeable membrane to achieve high selectivity for protein separations. This study presents a set of design equations and diagrams that describe the tradeoff between the yield and purification factor in HPCMP processes in terms of two parametric variables: the diffusive membrane selectivity and the ratio of the draw to bulk solution flow rates. Conditions are identified that provide the high yields and purification factors of interest in bioprocessing. In addition, hydrodynamic models for solute transport were used to evaluate the selectivity as a function of the membrane pore size distribution for purely size-based separations. Model calculations demonstrate that diffusive transport provides significantly greater selectivity than traditional pressure-driven membrane separations for the same pore size distribution due to differences in hindered transport rates for diffusion and convection. These results provide a framework that can be used for the development of HPCMP processes for highly selective protein separations.
This is the peer reviewed version of the following article: [Analysis of tradeoffs between purification factor and yield for high‐performance countercurrent membrane purification for protein separations. Biotechnology Progress 38, 1 (2021)], which has been published in final form at https://doi.org/10.1002/btpr.3221. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions: https://authorservices.wiley.com/author-resources/Journal-Authors/licensing/self-archiving.html#3.
|Work Title||Analysis of tradeoffs between purification factor and yield for high-performance countercurrent membrane purification for protein separations|
|License||CC BY-NC-ND 4.0 (Attribution-NonCommercial-NoDerivatives)|
|Publication Date||October 29, 2021|
|Publisher Identifier (DOI)||
|Deposited||January 23, 2023|
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