Architecture and dynamics of a novel desmosome-endoplasmic reticulum complex

The endoplasmic reticulum (ER) forms a dynamic network that contacts other cellular membranes to regulate stress responses, calcium signaling, and lipid transfer. Using high-resolution volume electron microscopy, we find that the ER forms a previously unknown association with keratin intermediate filaments and desmosomal cell-cell junctions. Peripheral ER assembles into mirror image-like arrangements at desmosomes and exhibits nanometer proximity to keratin filaments and the desmosome cytoplasmic plaque. ER tubules exhibit stable associations with desmosomes, and perturbation of desmosomes or keratin filaments alters ER organization, mobility, and expression of ER stress transcripts. These findings indicate that desmosomes and the keratin cytoskeleton regulate the distribution and function of the ER network. Overall, this study reveals a previously unknown subcellular architecture defined by the structural integration of ER tubules with an epithelial intercellular junction.

Files

Metadata

Work Title Architecture and dynamics of a novel desmosome-endoplasmic reticulum complex
Access
Penn State
Creators
  1. Navaneetha Krishnan Bharathan
Keyword
  1. Desmosome, Endosplasmic reticulum, keratin
License In Copyright (Rights Reserved)
Work Type Article
Publication Date 2023
Subject
  1. Cell Biology
Language
  1. English
DOI doi:10.26207/t3p7-zx04
Deposited February 01, 2023

Versions

Analytics

Collections

This resource is currently not in any collection.

Work History

Version 1
withdrawn

  • Created
  • Updated
  • Added Creator Navaneetha Krishnan Bharathan
  • Added Fig3C-DP-ER-Contact.xlsx
  • Updated License Show Changes
    License
    • https://rightsstatements.org/page/InC/1.0/
  • Published
  • Withdrawn
  • Updated