Affinity Electrophoresis for Analysis of Catalytic Module-Carbohydrate Interactions

Affinity electrophoresis has long been used to study the interaction between proteins and large soluble ligands. The technique has been found to have great utility for the examination of polysaccharide binding by proteins, particularly carbohydrate-binding modules (CBMs). In recent years carbohydrate surface binding sites of proteins, mostly enzymes, have also been investigated by this method. Here we describe a protocol for identifying binding interactions between enzyme catalytic modules and a variety of carbohydrate ligands.

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Work Title Affinity Electrophoresis for Analysis of Catalytic Module-Carbohydrate Interactions
Access
Open Access
Creators
  1. Darrell W. Cockburn
  2. Casper Wilkens
  3. Birte Svensson
Keyword
  1. Affinity electrophoresis
  2. Polyacrylamide gel electrophoresis
  3. Polysaccharide
  4. Surface binding site
  5. Carbohydrate binding module
  6. Dissociation constant
License In Copyright (Rights Reserved)
Work Type Article
Publisher
  1. Carbohydrate-Protein Interactions
Publication Date May 7, 2023
Publisher Identifier (DOI)
  1. https://doi.org/10.1007/978-1-0716-3151-5_6
Deposited March 21, 2024

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Version 1
published

  • Created

    March 21, 2024 15:23 by Researcher Metadata Database

  • Added Cockburn_2023_Methods_in_Molecular_Biology.pdf

    March 21, 2024 15:23 by Researcher Metadata Database

  • Added Creator Darrell W. Cockburn

    March 21, 2024 15:23 by Researcher Metadata Database

  • Added Creator Casper Wilkens

    March 21, 2024 15:23 by Researcher Metadata Database

  • Added Creator Birte Svensson

    March 21, 2024 15:23 by Researcher Metadata Database

  • Published

    March 21, 2024 15:23 by Researcher Metadata Database

  • Updated Keyword, Publisher Show Changes

    March 28, 2024 10:25 by avs5190

    Keyword
    • Affinity electrophoresis, Polyacrylamide gel electrophoresis, Polysaccharide, Surface binding site, Carbohydrate binding module, Dissociation constant
    Publisher
    • Methods in Molecular Biology
    • Carbohydrate-Protein Interactions
  • Updated

    April 04, 2024 10:22 by [unknown user]