Architecture and dynamics of a desmosome-endoplasmic reticulum complex
The endoplasmic reticulum (ER) forms a dynamic network that contacts other cellular membranes to regulate stress responses, calcium signaling, and lipid transfer. Using high-resolution volume electron microscopy, we find that the ER forms a previously unknown association with keratin intermediate filaments and desmosomal cell-cell junctions. Peripheral ER assembles into mirror image-like arrangements at desmosomes and exhibits nanometer proximity to keratin filaments and the desmosome cytoplasmic plaque. ER tubules exhibit stable associations with desmosomes, and perturbation of desmosomes or keratin filaments alters ER organization, mobility, and expression of ER stress transcripts. These findings indicate that desmosomes and the keratin cytoskeleton regulate the distribution and function of the ER network. Overall, this study reveals a previously unknown subcellular architecture defined by the structural integration of ER tubules with an epithelial intercellular junction.
|Architecture and dynamics of a desmosome-endoplasmic reticulum complex
|CC BY 4.0 (Attribution)
|February 01, 2023
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