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Created
July 21, 2022 12:38
by
Researcher Metadata Database
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Added
mAb_fouling_filter_morphology.docx
July 21, 2022 12:38
by
Researcher Metadata Database
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Added Creator Matthew Billups
July 21, 2022 12:38
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Researcher Metadata Database
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Added Creator Mirko Minervini
July 21, 2022 12:38
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Researcher Metadata Database
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Added Creator Melissa Holstein
July 21, 2022 12:38
by
Researcher Metadata Database
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Added Creator Hasin Feroz
July 21, 2022 12:38
by
Researcher Metadata Database
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Added Creator Swarnim Ranjan
July 21, 2022 12:38
by
Researcher Metadata Database
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Added Creator Jessica Hung
July 21, 2022 12:38
by
Researcher Metadata Database
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Added Creator Haiying Bao
July 21, 2022 12:38
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Researcher Metadata Database
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Added Creator Zheng Jian Li
July 21, 2022 12:38
by
Researcher Metadata Database
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Added Creator Sanchayita Ghose
July 21, 2022 12:38
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Researcher Metadata Database
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Added Creator Andrew L. Zydney
July 21, 2022 12:38
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Researcher Metadata Database
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Published
July 21, 2022 12:38
by
Researcher Metadata Database
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September 09, 2022 10:33
by
pmk5516
Description
Virus removal filtration is a critical step in the manufacture of monoclonal antibody products, providing a robust size-based removal of both enveloped and non-enveloped viruses. Many monoclonal antibodies show very large reductions in filtrate flux during virus filtration, with the mechanisms governing this behavior and its dependence on the properties of the virus filter and antibody remaining largely unknown. Experiments were performed using the highly asymmetric Viresolve® Pro and the relatively homogeneous Pegasus™ SV4 virus filters using a highly purified monoclonal antibody. The filtrate flux for a 4 g/L antibody solution through the Viresolve® Pro decreased by about 10-fold when the filter was oriented with the skin side down but by more than 1000-fold when the asymmetric filter orientation was reversed and used with the skin side up. The very large flux decline observed with the skin side up could be eliminated by placing a large pore size prefilter directly on top of the virus filter; this improvement in filtrate flux was not seen when the prefilter was used inline or as a batch prefiltration step. The increase in flux due to the prefilter was not related to the removal of large protein aggregates or to an alteration in the extent of concentration polarization. Instead, the prefilter appears to transiently disrupt reversible associations of the antibodies caused by strong intermolecular attractions. These results provide important insights into the role of membrane morphology and antibody properties on the filtrate flux during virus filtration.
- Virus removal filtration is a critical step in the manufacture of monoclonal antibody products, providing a robust size-based removal of both enveloped and non-enveloped viruses. Many monoclonal antibodies show very large reductions in filtrate flux during virus filtration, with the mechanisms governing this behavior and its dependence on the properties of the virus filter and antibody remaining largely unknown. Experiments were performed using the highly asymmetric Viresolve® Pro and the relatively homogeneous Pegasus™ SV4 virus filters using a highly purified monoclonal antibody. The filtrate flux for a 4 g/L antibody solution through the Viresolve® Pro decreased by about 10-fold when the filter was oriented with the skin side down but by more than 1000-fold when the asymmetric filter orientation was reversed and used with the skin side up. The very large flux decline observed with the skin side up could be eliminated by placing a large pore size prefilter directly on top of the virus filter; this improvement in filtrate flux was not seen when the prefilter was used inline or as a batch prefiltration step. The increase in flux due to the prefilter was not related to the removal of large protein aggregates or to an alteration in the extent of concentration polarization. Instead, the prefilter appears to transiently disrupt reversible associations of the antibodies caused by strong intermolecular attractions. These results provide important insights into the role of membrane morphology and antibody properties on the filtrate flux during virus filtration.
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September 09, 2022 10:35
by
pmk5516
Work Title
Role of membrane structure on the filtrate flux during monoclonal antibody filtration through virus retentive membranes
- ! Role of membrane structure on the filtrate flux during monoclonal antibody filtration through virus retentive membranes
Keyword
- Virus filtration, Monoclonal antibody, Fouling, Ultrafiltration, Membrane structure
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May 24, 2023 14:00
by
pmk5516
Work Title
! Role of membrane structure on the filtrate flux during monoclonal antibody filtration through virus retentive membranes
- Role of membrane structure on the filtrate flux during monoclonal antibody filtration through virus retentive membranes
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Updated
April 04, 2024 10:21
by
[unknown user]