3_Mapping.bam

This project is to study the heat effect on RNA structure change in rice. The wild-type rice were grown in greenhouse and shoot tissue of two-week-old plant was used for library preparation. 1 g of shoot tissue were cut off the plant right before each treatment. After following our previous pipeline of Structure-seq for +DMS treatment, the -DMS sample was processed with no DMS applied as the control. For the heat parallel experiment, -DMS and +DMS samples were prepared. Library preparation was following our previous library construction pipeline with some major changes and optimization. All 12 individual biological libraries was done with unique Illumina TruSeq barcode and been sequenced by HiSeq2500.

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Work Title 3_Mapping.bam
Access
Open Access
Creators
  1. Zhao Su
  2. Yin Tang
  3. Sarah Assmann
  4. Philip Bevilacqua
Keyword
  1. Genomics
  2. RNA
  3. Oryza sativa
License Attribution 3.0 United States
Work Type Dataset
Subject
  1. Functional genomics
  2. Molecular genetics
Publisher Identifier (DOI)
  1. http://doi.org/10.5072/FK2XD0X34X
Deposited November 11, 2016

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Version 1
published

  • Created
  • Added j3t945s237_version1_3_Mapping.bam
  • Added Creator Zhao Su
  • Added Creator Yin Tang
  • Added Creator Sarah Assmann
  • Added Creator Philip Bevilacqua
  • Published