Manuscript for "Genome-Wide Surveillance of Genital Herpes Simplex Virus Type 1 from Multiple Anatomic Sites over Time"

This is the manuscript file -- with supplemental data attached inside the PDF -- for this citation: Shipley, Mackenzie M, Daniel W Renner, Mariliis Ott, David C Bloom, David M Koelle, Christine Johnston, and Moriah L Szpara. “Genome-Wide Surveillance of Genital Herpes Simplex Virus Type 1 from Multiple Anatomic Sites over Time.” The Journal of Infectious Diseases 218, no. 4 (June 18, 2018): 595–605. https://doi.org/10.1093/infdis/jiy216.

Abstract: Here we present genomic and in vitro analyses of temporally separated episodes of herpes simplex virus type 1 (HSV-1) shedding by an HSV-1–seropositive and human immunodeficiency virus (HIV)/HSV-2–seronegative individual who has frequent recurrences of genital HSV-1. Using oligonucleotide enrichment, we compared viral genomes from uncultured swab specimens collected on different days and from distinct genital sites. We found that viral genomes from 7 swab specimens and 3 cultured specimens collected over a 4-month period from the same individual were 98.5% identical. We observed a >2-fold difference in the number of minority variants between swab specimens from lesions, swab specimens from nonlesion sites, and cultured specimens. This virus appeared distinct in its phylogenetic relationship to other strains, and it contained novel coding variations in 21 viral proteins. This included a truncation in the UL11 tegument protein, which is involved in viral egress and spread. Normal immune responses were identified, suggesting that unique viral genomic features may contribute to the recurrent genital infection that this participant experiences.

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Work Title Manuscript for "Genome-Wide Surveillance of Genital Herpes Simplex Virus Type 1 from Multiple Anatomic Sites over Time"
Access
Open Access
Creators
  1. Mackenzie M. Shipley
  2. Daniel W. Renner
  3. Mariliis Ott
  4. David C. Bloom
  5. David M. Koelle
  6. Christine Johnston
  7. Moriah L. Szpara
Keyword
  1. HSV-1
  2. Genomes
  3. Genital
  4. In vivo
  5. Viral diversity
  6. Herpes simplex virus 1
  7. Clinical
  8. High-throughput sequencing
  9. Oligonucleotide enrichment
  10. Comparative genomics
License All rights reserved
Work Type Article
Publisher
  1. The Journal of Infectious Diseases
Publication Date June 18, 2018
Publisher Identifier (DOI)
  1. https://doi.org/10.1093/infdis/jiy216
Related URLs
  1. https://scholarsphere.psu.edu/resources/7af7a69c-91e3-44ad-83d9-52ca3579e6b6
Deposited August 09, 2019

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Version 1
published

  • Created

    November 16, 2020 22:03 by Scholarsphere 4 Migration

  • Added Shipley18_JID_v29_w_SUPP.pdf

    November 16, 2020 22:03 by Scholarsphere 4 Migration

  • Added Creator MORIAH LOUISE SZPARA

    November 16, 2020 22:03 by Scholarsphere 4 Migration

  • Published

    November 16, 2020 22:03 by Scholarsphere 4 Migration

  • Updated Work Title, Keyword, Publisher, and 3 more Show Changes

    February 29, 2024 13:21 by avs5190

    Work Title
    • Shipley18_JID_v29_w_SUPP.pdf
    • Genome-Wide Surveillance of Genital Herpes Simplex Virus Type 1 from Multiple Anatomic Sites over Time
    Keyword
    • HSV-1, genomes, genital, in vivo, viral diversity, herpes simplex virus 1
    • HSV-1, Genomes, Genital, In vivo, Viral diversity, Herpes simplex virus 1, Clinical, High-throughput sequencing, Oligonucleotide enrichment, Comparative genomics
    Publisher
    • The Journal of Infectious Diseases
    Publisher Identifier (DOI)
    • https://doi.org/10.1093/infdis/jiy216
    Related URLs
    • https://scholarsphere.psu.edu/resources/7af7a69c-91e3-44ad-83d9-52ca3579e6b6
    Publication Date
    • 2018-06-18
  • Renamed Creator Moriah L. Szpara Show Changes

    February 29, 2024 13:22 by avs5190

    • MORIAH LOUISE SZPARA
    • Moriah L. Szpara
  • Added Creator Mackenzie M. Shipley

    February 29, 2024 13:22 by avs5190

  • Added Creator Daniel W. Renner

    February 29, 2024 13:22 by avs5190

  • Added Creator Mariliis Ott

    February 29, 2024 13:22 by avs5190

  • Added Creator David C. Bloom

    February 29, 2024 13:22 by avs5190

  • Added Creator David M. Koelle

    February 29, 2024 13:22 by avs5190

  • Added Creator Christine Johnston

    February 29, 2024 13:22 by avs5190

  • Updated Description Show Changes

    February 29, 2024 13:23 by avs5190

    Description
    • This is the manuscript file -- with supplemental data attached inside the PDF -- for this citation: Shipley, Mackenzie M, Daniel W Renner, Mariliis Ott, David C Bloom, David M Koelle, Christine Johnston, and Moriah L Szpara. “Genome-Wide Surveillance of Genital Herpes Simplex Virus Type 1 from Multiple Anatomic Sites over Time.” The Journal of Infectious Diseases 218, no. 4 (June 18, 2018): 595–605. https://doi.org/10.1093/infdis/jiy216.
    • This is the manuscript file -- with supplemental data attached inside the PDF -- for this citation: Shipley, Mackenzie M, Daniel W Renner, Mariliis Ott, David C Bloom, David M Koelle, Christine Johnston, and Moriah L Szpara. “Genome-Wide Surveillance of Genital Herpes Simplex Virus Type 1 from Multiple Anatomic Sites over Time.” The Journal of Infectious Diseases 218, no. 4 (June 18, 2018): 595–605. https://doi.org/10.1093/infdis/jiy216.
    • Abstract: Here we present genomic and in vitro analyses of temporally separated episodes of herpes simplex virus type 1 (HSV-1) shedding by an HSV-1–seropositive and human immunodeficiency virus (HIV)/HSV-2–seronegative individual who has frequent recurrences of genital HSV-1. Using oligonucleotide enrichment, we compared viral genomes from uncultured swab specimens collected on different days and from distinct genital sites. We found that viral genomes from 7 swab specimens and 3 cultured specimens collected over a 4-month period from the same individual were 98.5% identical. We observed a >2-fold difference in the number of minority variants between swab specimens from lesions, swab specimens from nonlesion sites, and cultured specimens. This virus appeared distinct in its phylogenetic relationship to other strains, and it contained novel coding variations in 21 viral proteins. This included a truncation in the UL11 tegument protein, which is involved in viral egress and spread. Normal immune responses were identified, suggesting that unique viral genomic features may contribute to the recurrent genital infection that this participant experiences.
  • Updated Work Title Show Changes

    February 29, 2024 13:24 by avs5190

    Work Title
    • Genome-Wide Surveillance of Genital Herpes Simplex Virus Type 1 from Multiple Anatomic Sites over Time
    • Manuscript for "Genome-Wide Surveillance of Genital Herpes Simplex Virus Type 1 from Multiple Anatomic Sites over Time"
  • Updated

    April 04, 2024 10:19 by [unknown user]