Passive Immunization with a Single Monoclonal Neutralizing Antibody Protects against Cutaneous and Mucosal Mouse Papillomavirus Infections

We have established a mouse papillomavirus (MmuPV1) model that induces both cutaneous and mucosal infections and cancers. In the current study, we use this model to test our hypothesis that passive immunization using a single neutralizing monoclonal antibody can protect both cutaneous and mucosal sites at different time points after viral inoculation. We conducted a series of experiments involving the administration of either a neutralizing monoclonal antibody, MPV.A4, or control monoclonal antibodies to both outbred and inbred athymic mice. Three clinically relevant mucosal sites (lower genital tract for females and anus and tongue for both males and females) and two cutaneous sites (muzzle and tail) were tested. At the termination of the experiments, all tested tissues were harvested for virological analyses. Significantly lower levels of viral signals were detected in the MPV.A4-treated female mice up to 6 h post-viral inoculation compared to those in the isotype control. Interestingly, males displayed partial protection when they received MPV.A4 at the time of viral inoculation, even though they were completely protected when receiving MPV.A4 at 24 h before viral inoculation. We detected MPV.A4 in the blood starting at 1 h and up to 8 weeks postadministration in some mice. Parallel to these in vivo studies, we conducted in vitro neutralization using a mouse keratinocyte cell line and observed complete neutralization up to 8 h post-viral inoculation. Thus, passive immunization with a monoclonal neutralizing antibody can protect against papillomavirus infection at both cutaneous and mucosal sites and is time dependent.


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Work Title Passive Immunization with a Single Monoclonal Neutralizing Antibody Protects against Cutaneous and Mucosal Mouse Papillomavirus Infections
Open Access
  1. Sarah A. Brendle
  2. Jingwei Li
  3. Nancy M. Cladel
  4. Karla K. Balogh
  5. Jennifer Booth
  6. Debra A. Shearer
  7. Vonn Walter
  8. Song Lu
  9. Neil D. Christensen
  10. Danielle Covington
  11. Jake DeBroff
  12. Janice Milici
  13. Yusheng Zhu
  14. Raphael Viscidi
  15. Jiafen Hu
License In Copyright (Rights Reserved)
Work Type Article
  1. Journal of Virology
Publication Date August 1, 2022
Publisher Identifier (DOI)
Deposited January 30, 2023




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Work History

Version 1

  • Created
  • Added jvi.00703-22.pdf
  • Added Creator Sarah A. Brendle
  • Added Creator Jingwei Li
  • Added Creator Nancy M. Cladel
  • Added Creator Karla K. Balogh
  • Added Creator Jennifer Booth
  • Added Creator Debra A. Shearer
  • Added Creator Vonn Walter
  • Added Creator Song Lu
  • Added Creator Neil D. Christensen
  • Added Creator Danielle Covington
  • Added Creator Jake DeBroff
  • Added Creator Janice Milici
  • Added Creator Yusheng Zhu
  • Added Creator Raphael Viscidi
  • Added Creator Jiafen Hu
  • Published