Modified intermediate pore blockage model describes fouling behavior during sterile filtration of glycoconjugate vaccines

Sterile filtration using 0.2 µm pore size membranes is used to insure sterility of almost all biopharmaceuticals. Sterile filtration can be particularly challenging with large complex molecules such as glycoconjugate vaccines that are nearly 200 nm in size, leading to low yield and capacity. The objective of this study was to develop a fundamental understanding of the fouling behavior during sterile filtration of a glycoconjugate vaccine through the Durapore® PVDF sterile filtration membrane. Confocal microscopy with a fluorescently-labeled glycoconjugate demonstrated that fouling was limited to a thin region near the entrance of the homogeneous membrane. A modified intermediate pore blockage model was developed to describe the rate of flux decline accounting for the fouling at the pore entrance and the highly interconnected pore structure of the Durapore® membrane. Model calculations were in very good agreement with the fouling data during both constant pressure and constant flux filtration. These results provide important insights into the fouling behavior during sterile filtration of glycoconjugate vaccines that are used to provide immunization against significant bacterial infections.

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Work Title Modified intermediate pore blockage model describes fouling behavior during sterile filtration of glycoconjugate vaccines
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Open Access
Creators
  1. Parinaz Emami
  2. Fatemeh Fallahianbijan
  3. Erica J Dinse
  4. Seyed Pouria Motevalian
  5. Brenda Carrillo Conde
  6. Kelvin Reilly
  7. Andrew Zydney
Keyword
  1. Sterile filtration, Fouling, Glycoconjugate, Vaccine, Pore blockage
License CC BY-NC-ND 4.0 (Attribution-NonCommercial-NoDerivatives)
Work Type Article
Publisher
  1. Elsevier
Publication Date July 22, 2020
Publisher Identifier (DOI)
  1. 10.1016/j.memsci.2020.118495
Deposited March 07, 2021

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Version 1
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  • Created
  • Added Creator Parinaz Emami
  • Added Creator Fatemeh Fallahianbijan
  • Added Creator Erica J Dinse
  • Added Creator Seyed Pouria Motevalian
  • Added Creator Brenda Carrillo Conde
  • Added Creator Kelvin Reilly
  • Added Creator Andrew Zydney
  • Added Glycoconjugate sterile filtration-Revised.docx
  • Updated License Show Changes
    License
    • https://creativecommons.org/licenses/by-nc-nd/4.0/
  • Published
  • Updated
  • Updated