Data sets for "A broad survey of choanoflagellates revises the evolutionary history of the Shaker family of voltage-gated K+ channels in animals"

Electrophysiology, Molecular Dynamics and sequence data sets for "A broad survey of choanoflagellates revises the evolutionary history of the Shaker family of voltage-gated K+ channels in animals", in press at PNAS

ABSTRACT The Shaker family of voltage-gated K+ channels has been thought of as an animal-specific ion channel family that diversified in concert with nervous systems. It comprises four functionally independent gene subfamilies (Kv1-4) that encode diverse neuronal K+ currents. Comparison of animal genomes predicts that only the Kv1 subfamily was present in the animal common ancestor. Here we show that some choanoflagellates, the closest protozoan sister lineage to animals, also have Shaker family K+ channels. Choanoflagellate Shaker family channels are surprisingly most closely related to the animal Kv2-4 subfamilies which were believed to have evolved only after the divergence of ctenophores and sponges from cnidarians and bilaterians. Structural modelling predicts that the choanoflagellate channels share a T1 Zn2+ binding site with Kv2-4 channels that is absent in Kv1 channels. We functionally expressed three Shakers from Salpingoeca helianthica (SheliKvT1.1-3) in Xenopus oocytes. SheliKvT1.1-3 function only in two heteromultimeric combinations (SheliKvT1.1/1.2 and SheliKvT1.1/1.3) and encode fast N-type inactivating K+ channels with distinct voltage dependence that are most similar to the widespread animal Kv1-encoded A-type Shakers. Structural modelling of the T1 assembly domain supports a preference for heteromeric assembly in a 2:2 stoichiometry. These results push the origin of the Shaker family back into a common ancestor of metazoans and choanoflagellates. They also suggest that the animal common ancestor had at least two distinct molecular lineages of Shaker channels, a Kv1 subfamily lineage predicted from comparison of animal genomes and a Kv2-4 lineage predicted from comparison of animals and choanoflagellates.

Citation

Jegla, Timothy; Simonson, Benjamin (2024). Data sets for "A broad survey of choanoflagellates revises the evolutionary history of the Shaker family of voltage-gated K+ channels in animals" [Data set]. Scholarsphere. https://doi.org/10.26207/4zct-nc59

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Work Title Data sets for "A broad survey of choanoflagellates revises the evolutionary history of the Shaker family of voltage-gated K+ channels in animals"
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Open Access
Creators
  1. Timothy Jegla
  2. Benjamin Thomas Simonson
License CC0 1.0 (Public Domain Dedication)
Work Type Dataset
Publication Date July 2024
DOI doi:10.26207/4zct-nc59
Deposited July 05, 2024

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    Description
    • Electrophysiology, Molecular Dynamics and sequence data sets for "A broad survey of choanoflagellates revises the evolutionary history of the Shaker family of voltage-gated K+ channels in animals", in press at PNAS
    • ABSTRACT
    • The Shaker family of voltage-gated K+ channels has been thought of as an animal-specific ion channel family that diversified in concert with nervous systems. It comprises four functionally independent gene subfamilies (Kv1-4) that encode diverse neuronal K+ currents. Comparison of animal genomes predicts that only the Kv1 subfamily was present in the animal common ancestor. Here we show that some choanoflagellates, the closest protozoan sister lineage to animals, also have Shaker family K+ channels. Choanoflagellate Shaker family channels are surprisingly most closely related to the animal Kv2-4 subfamilies which were believed to have evolved only after the divergence of ctenophores and sponges from cnidarians and bilaterians. Structural modelling predicts that the choanoflagellate channels share a T1 Zn2+ binding site with Kv2-4 channels that is absent in Kv1 channels. We functionally expressed three Shakers from Salpingoeca helianthica (SheliKvT1.1-3) in Xenopus oocytes. SheliKvT1.1-3 function only in two heteromultimeric combinations (SheliKvT1.1/1.2 and SheliKvT1.1/1.3) and encode fast N-type inactivating K+ channels with distinct voltage dependence that are most similar to the widespread animal Kv1-encoded A-type Shakers. Structural modelling of the T1 assembly domain supports a preference for heteromeric assembly in a 2:2 stoichiometry. These results push the origin of the Shaker family back into a common ancestor of metazoans and choanoflagellates. They also suggest that the animal common ancestor had at least two distinct molecular lineages of Shaker channels, a Kv1 subfamily lineage predicted from comparison of animal genomes and a Kv2-4 lineage predicted from comparison of animals and choanoflagellates.
    Publication Date
    • 2024-07
  • Added Creator Timothy Jegla
  • Added Creator Benjamin Thomas Simonson
  • Added Electrophysiology Data and Analysis.zip
  • Added Sheli_KvT1_1_EV.gb
  • Added Sheli_KvT1_2_EV.gb
  • Added Sheli_KvT1_3_EV.gb
  • Updated License Show Changes
    License
    • http://creativecommons.org/publicdomain/zero/1.0/
  • Added Choanoflagellate Models.zip
  • Added Readme.txt
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