-
Created
March 18, 2024 12:03
by
Researcher Metadata Database
-
Added
2023-PMS-Cheng-Peroxiredoxin_1_transfer_during_mating_protects_eupyrene_sperm_against_oxdative_stress.pdf
March 18, 2024 12:03
by
Researcher Metadata Database
-
Added Creator Jie Cheng
March 18, 2024 12:03
by
Researcher Metadata Database
-
Added Creator Lin Zhu
March 18, 2024 12:03
by
Researcher Metadata Database
-
Added Creator Fang Zhu
March 18, 2024 12:03
by
Researcher Metadata Database
-
Added Creator Peng Zhao
March 18, 2024 12:03
by
Researcher Metadata Database
-
Added Creator Qiong Xi Li
March 18, 2024 12:03
by
Researcher Metadata Database
-
Added Creator Zi Hang Lu
March 18, 2024 12:03
by
Researcher Metadata Database
-
Added Creator Song Dou Zhang
March 18, 2024 12:03
by
Researcher Metadata Database
-
Added Creator Zhen Li
March 18, 2024 12:03
by
Researcher Metadata Database
-
Added Creator Xiao Xia Liu
March 18, 2024 12:03
by
Researcher Metadata Database
-
Published
March 18, 2024 12:03
by
Researcher Metadata Database
-
March 19, 2024 10:11
by
avs5190
Keyword
- Oriental fruit moth, Peroxiredoxin 1, Mating, Fertility, Oxidative stress, Sperm quality
Description
<p>BACKGROUND: Each Grapholita molesta female only copulates once during its lifetime and thus must maintain the viability of stored eupyrene sperm for male reproductive success. The male ejaculate comprises abundant accessory gland proteins produced by the male accessory gland (AG), and many of which are major effectors for sperm storage and maintenance. RESULTS: Here, we reported that an antioxidant protein, peroxiredoxin 1 (GmolPrx1), secreted by the male AG, is essential for protecting eupyrene sperm from oxidative stress and maintaining their quality during storage in the female bursa copulatrix (BC). Our data showed that GmolPrx1 is highly expressed in the AG of sexually mature males. The GmolPrx1 protein is localized to the cytoplasm of AG cells and delivered to the female BC during mating. Knockdown of GmolPrx1 strongly decreased the fertility of mated females. Additionally, we evaluated oxidative status in the spermatophore of females and found that the content of hydrogen peroxide increased significantly after mating with GmolPrx1 knockdown males. Finally, the quality assessment of eupyrene sperm demonstrated that the plasma membrane integrity, acrosome integrity, and DNA integrity were all severely impaired in the spermatophore of females after mating with GmolPrx1 knockdown males, which may contribute to the fertility decline in males. CONCLUSION: Our current data demonstrated that activities of eupyrene sperm stored in females can be significantly impaired by enhanced oxidative stress through knocking down of GmolPrx1 in males. Our finding thus may further lay new foundations for the control of G. molesta through suppressing their populations by manipulating male reproductive genes.</p>
- <p>BACKGROUND: Each _Grapholita molesta_ female only copulates once during its lifetime and thus must maintain the viability of stored eupyrene sperm for male reproductive success. The male ejaculate comprises abundant accessory gland proteins produced by the male accessory gland (AG), and many of which are major effectors for sperm storage and maintenance.
- RESULTS: Here, we reported that an antioxidant protein, peroxiredoxin 1 (GmolPrx1), secreted by the male AG, is essential for protecting eupyrene sperm from oxidative stress and maintaining their quality during storage in the female bursa copulatrix (BC). Our data showed that GmolPrx1 is highly expressed in the AG of sexually mature males. The GmolPrx1 protein is localized to the cytoplasm of AG cells and delivered to the female BC during mating. Knockdown of _GmolPrx1_ strongly decreased the fertility of mated females. Additionally, we evaluated oxidative status in the spermatophore of females and found that the content of hydrogen peroxide increased significantly after mating with _GmolPrx1_ knockdown males. Finally, the quality assessment of eupyrene sperm demonstrated that the plasma membrane integrity, acrosome integrity, and DNA integrity were all severely impaired in the spermatophore of females after mating with _GmolPrx1_ knockdown males, which may contribute to the fertility decline in males.
- CONCLUSION: Our current data demonstrated that activities of eupyrene sperm stored in females can be significantly impaired by enhanced oxidative stress through knocking down of _GmolPrx1_ in males. Our finding thus may further lay new foundations for the control of _G. molesta_ through suppressing their populations by manipulating male reproductive genes.</p>
Publication Date
-
March 19, 2024 10:11
by
avs5190
-
March 19, 2024 10:11
by
avs5190
-
March 19, 2024 10:11
by
avs5190
Song Dou Zhang
- Song-Dou Zhang
-
March 19, 2024 10:11
by
avs5190
Xiao Xia Liu
- Xiao-Xia Liu
-
Updated
April 04, 2024 10:22
by
[unknown user]