Metabolites imaging in single HeLa cells using GCIB-SIMS Public

Metabolons, multiprotein complexes consisting of sequential enzymes of a metabolic pathway, are proposed to be biosynthetic ‘hot-spots’ within the cell. However, experimental demonstration of their presence and features of their function has remained challenging. We used metabolomics and in-situ three-dimensional sub-micron chemical imaging of single cells by gas cluster ion beam secondary ion mass spectrometry (GCIB-SIMS) to directly visualize de novo purine biosynthesis (DNPB) by a multienzyme complex, the purinosome. We found that purinosomes comprise nine enzymes that act synergistically, channeling the pathway intermediates to synthesize purine nucleotides, increasing the pathway flux, and influencing the AMP:GMP ratio. Our work also highlights application of high-resolution GCIB-SIMS for multiplexed biomolecular analysis at the level of single cells.

README

File list:

  • 01_22_18 HeLa P+12C 70kV CO2 12k neg 01.zip
  • 03_05_18 HeLa P-12C Gly 70KV CO2 14k neg 01.zip
  • 05_09_18 HeLa C13 P- 70kV CO2 13k neg 01.zip
  • 05_09_18 HeLa N15 P- 70kV CO2 13k neg.zip
  • 12_04_18 HeLa knockout 15N 70kv CO214k neg.zip
  • 12_20_17 HeLa P+13C 70kv CO2 12k neg.zip

Additional notes:

The zip files contain raw instrument data that may be opened with Ionoptika Image Analyser (v1.0.2.3). Software download and temporary license request can be made via email support@ionoptika.co.uk. Email the authors if there are any further questions regarding the data processing.

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User Vidhi Pareek has attached 12_04_18 HeLa knockout 15N 70kv CO214k neg.zip to Metabolites imaging in single HeLa cells using GCIB-SIMS about 1 month ago
User Vidhi Pareek has attached 01_22_18 HeLa P+12C 70kV CO2 12k neg 01.zip to Metabolites imaging in single HeLa cells using GCIB-SIMS about 1 month ago
User Vidhi Pareek has attached 05_09_18 HeLa C13 P- 70kV CO2 13k neg 01.zip to Metabolites imaging in single HeLa cells using GCIB-SIMS about 1 month ago
User Vidhi Pareek has attached 12_20_17 HeLa P+13C 70kv CO2 12k neg.zip to Metabolites imaging in single HeLa cells using GCIB-SIMS about 1 month ago
User Vidhi Pareek has attached 05_09_18 HeLa N15 P- 70kV CO2 13k neg.zip to Metabolites imaging in single HeLa cells using GCIB-SIMS about 1 month ago
User Vidhi Pareek has attached 03_05_18 HeLa P-12C Gly 70KV CO2 14k neg 01.zip to Metabolites imaging in single HeLa cells using GCIB-SIMS about 1 month ago
User Vidhi Pareek has attached 03_05_18 HeLa P-12C Gly 70KV CO2 14k neg 01.zip to Metabolites imaging in single HeLa cells using GCIB-SIMS about 1 month ago
User Seth R Erickson has updated Metabolites imaging in single HeLa cells using GCIB-SIMS about 1 month ago
User Seth R Erickson has updated Metabolites imaging in single HeLa cells using GCIB-SIMS about 1 month ago
User Seth R Erickson has updated Metabolites imaging in single HeLa cells using GCIB-SIMS about 1 month ago
User Seth R Erickson has attached README.txt to Metabolites imaging in single HeLa cells using GCIB-SIMS about 1 month ago
User Seth R Erickson has deposited Metabolites imaging in single HeLa cells using GCIB-SIMS about 1 month ago