Prebiotically-relevant low polyion multivalency can improve functionality of membraneless compartments Public

Multivalent polyions can undergo complex coacervation, producing membraneless compartments that accumulate ribozymes and enhance catalysis, and offering a mechanism for functional prebiotic compartmentalization in the origins of life. Here, we evaluate the impact of lower, more prebiotically-relevant, polyion multivalency on the functional performance of coacervates as compartments. Positively and negatively charged homopeptides with 1 – 100 residues and adenosine mono-, di-, and triphosphate nucleotides are used as model polyions. Polycation/polyanion pairs are tested for coacervation, and resulting membraneless compartments are analyzed for salt resistance, ability to provide a distinct internal microenvironment (apparent local pH, RNA partitioning), and effect on RNA structure formation. We find that coacervates formed by phase separation of the shorter polyions more effectively generated distinct pH microenvironments, accumulated RNA, and preserved duplexes than those formed by longer polyions. Hence, coacervates formed by reduced multivalency polyions are not only viable as functional compartments for prebiotic chemistries, they can outperform higher molecular weight analogues.

README

General Information
Title:
Lipid Vesicle-Coated Complex Coacervates
Author Information:
* Corresponding Author: Christine D. Keating and Philip C. Bevilacqua
* Co- author: Fatma Pir Cakmak, Saehyun Choi, McCauley O. Meyer, Philip C. Bevilacqua and Christine D. Keating
* Contact(s): cmd8@psu.edu
Date of data collection/creation:
2020-10-15
Geographic location of data collection:
University Park, PA, The USA
Funders and sponsors of data collection:
This work was supported by the NASA Exobiology program grant 80NSSC17K0034. S. C. was supported by Future Investigators in NASA Earth and Space Science and Technology (FINESST) under Grant 80NSSC19K1531.
License & restrictions on data reuse:
Creative Commons Attribution 0.0 International (CC BY 0.0)
Related publications:
" Prebiotically-relevant low polyion multivalency can improve functionality of membraneless compartments". Fatma Pir Cakmak, Saehyun Choi, McCauley O. Meyer, Philip C. Bevilacqua and Christine D. Keating
bioRxiv, February 2020, 35, 24, 7830-7840; DOI: https://doi.org/10.1101/2020.02.23.961920
Links to other publicly accessible locations of the data:
All data for all figure, supporting figures, and tables are available at https://doi.org/10.1101/2020.02.23.961920

Data & File Overview
File list:
* Final-Pathlength corrected turbidity K D Nts.xlsx: raw data for turbidity in the main texts Figure 3 for Lysine.
* Final-Pathlength corrected turbidity R D Nts.xlsx: raw data for turbidity in the main texts Figure 3 for Arginine.
* Ratio comparion- Final Rev2.xlsx: raw data for fluorescence ratio comparison in the main texts Figure 3.
* ssRNA10contconcentrationandcalibration.xlsx: raw data for continuous phase concentration calculation in the main text Figure 4.
* ssRNA20
contconcentrationandcalibration.xlsx: raw data for continuous phase concentration calculation in the main text Figure 4.
* ConcentrationindropletsssR20merwithcalibration.xlsx: raw data for droplet phase concentration calculation in the main text Figure 4.
* Concentration
indropletsssR10withcalibration.xlsx: raw data for droplet phase concentration calculation in the main text Figure 4.
* Both
calibrationandconcincontdsR10.xlsx: raw data for continuous phase concentration calculation in the main text Figure 4.
* BothcalibrationandconcincontdsR20.xlsx: raw data for continuous phase concentration calculation in the main text Figure 4.
* Concentration
indropletsdsR20withcalibrationcurves.xlsx: raw data for droplet phase concentration calculation in the main text Figure 4.
* Concentration
indropletsdsR10withcalibrationcurve.xlsx: raw data for droplet phase concentration calculation in the main text Figure 4.
* Asp100Lys100ssR10_1.2-1.xlsx: raw data for droplet phase concentration calculation in the SI Figure 6.
* Asp100-Lys100
ssR201-2-1.xlsx: raw data for droplet phase concentration calculation in the SI Figure 6.
* ds
R10Asp30Lys30FRETCalculationsCoacervateS1.xlsx: raw data for FRET data Asp30-Lys30 ds RNA trial 1 in the main texts Figure 5.
* dsR10Asp30Lys30FRETCalculationsCoacervateS2.xlsx: raw data for FRET data Asp30-Lys30 ds RNA trial 2 in the main texts Figure 5.
* ds
R10Asp30Lys30FRETCalculationsCoacervateS3.xlsx: raw data for FRET data Asp30-Lys30 ds RNA trial 3 in the main texts Figure 5.
* Asp 30- Lys 30 ss RNA 10 FRET Calculations Coacervate S3.xlsx: raw data for FRET data Asp30-Lys30 ss RNA trial 3 in the main texts Figure 5.
* Asp 30- Lys 30 ss RNA 10 FRET Calculations Coacervate S2.xlsx: raw data for FRET data Asp30-Lys30 ss RNA trial 2 in the main texts Figure 5.
* Asp 30- Lys 30 ss RNA 10 FRET Calculations Coacervate S1.xlsx: raw data for FRET data Asp30-Lys30 ss RNA trial 1 in the main texts Figure 5.
* ATP Lys 10 ss RNA 10 FRET Calculations Coacervate S2.xlsx: raw data for FRET data ATP-Lys10 ss RNA trial 2 in the main texts Figure 5.
* ATP Lys 10 ss RNA 10 FRET Calculations Coacervate S3.xlsx: raw data for FRET data ATP-Lys10 ss RNA trial 3 in the main texts Figure 5.
* ATP Lys 10 ss RNA 10 FRET Calculations Coacervate S1.xlsx: raw data for FRET data ATP-Lys10 ss RNA trial 1 in the main texts Figure 5.
* ds R10 10 uM 1X salt control repeat S1.xlsx: raw data for FRET data in buffer ds RNA trial 1 in the main texts Figure 5.
* ds R10 10 uM 1X salt control repeat S2.xlsx: raw data for FRET data in buffer ds RNA trial 2 in the main texts Figure 5.
* ds R10 10 uM 1X salt control repeat S3.xlsx: raw data for FRET data in buffer ds RNA trial 3 in the main texts Figure 5.
* ds R10 Asp 10 Lys 10 FRET Calculations Coacervate S1.xlsx: raw data for FRET data in Asp 10-Lys 10 ds RNA trial 1 in the main texts Figure 5.
* ds R10 Asp 10 Lys 10 FRET Calculations Coacervate S2.xlsx: raw data for FRET data in Asp 10-Lys 10 ds RNA trial 2 in the main texts Figure 5.
* ds R10 Asp 10 Lys 10 FRET Calculations Coacervate S3.xlsx: raw data for FRET data in Asp 10-Lys 10 ds RNA trial 3 in the main texts Figure 5.
* ds R10 Asp 30 Lys 30 FRET Calculations Coacervate S1.xlsx: raw data for FRET data in Asp 30-Lys 30 ds RNA trial 1 in the main texts Figure 5.
* ds R10 Asp 30 Lys 30 FRET Calculations Coacervate S2.xlsx: raw data for FRET data in Asp 30-Lys 30 ds RNA trial 2 in the main texts Figure 5.
* ds R10 Asp 30 Lys 30 FRET Calculations Coacervate S3.xlsx: raw data for FRET data in Asp 30-Lys 30 ds RNA trial 3 in the main texts Figure 5.
* ds R10 Asp 100 Lys 100 1-1.2 FRET Coacervate S1.xlsx: raw data for FRET data in Asp 100-Lys 100 ds RNA trial 1 in the main texts Figure 5.
* ds R10 Asp 100 Lys 100 1-1.2 FRET Coacervate S2.xlsx: raw data for FRET data in Asp 100-Lys 100 ds RNA trial 2 in the main texts Figure 5.
* ds R10 Asp 100 Lys 100 1-1.2 FRET Coacervate S3.xlsx: raw data for FRET data in Asp 100-Lys 100 ds RNA trial 3 in the main texts Figure 5.
* ds R10 ATP Lys 10 FRET Calculations Coacervate S1.xlsx: raw data for FRET data in ATP-Lys 10 ds RNA trial 1 in the main texts Figure 5.
* ds R10 ATP Lys 10 FRET Calculations Coacervate S2.xlsx: raw data for FRET data in ATP-Lys 10 ds RNA trial 2 in the main texts Figure 5.
* ds R10 ATP Lys 10 FRET Calculations Coacervate S3.xlsx: raw data for FRET data in ATP-Lys 10 ds RNA trial 3 in the main texts Figure 5.
* ss R10 10 uM control S1.xlsx: raw data for FRET data in buffer 10 ss RNA trial 1 in the main texts Figure 5.
* ss R10 10 uM control S2.xlsx: raw data for FRET data in buffer 10 ss RNA trial 2 in the main texts Figure 5.
* ss R10 10 uM control S3.xlsx: raw data for FRET data in buffer 10 ss RNA trial 3 in the main texts Figure 5.
* Lys10-Asp10T1-3spectrum.xlsx: raw data for Figure S4.
* Lys10-Asp10T1-3spectrum2.xlsx: raw data for Figure S4.
* Lys10-ATPK10-D5Spectrum.xlsx: raw data for Figure S4.
* Lys30-Asp5T1-3spectrum.xlsx: raw data for Figure S4.
* Lys30-Asp30T1-3spectrum.xlsx: raw data for Figure S4.
* Lys100-Asp100T1-3spectrum.xlsx: raw data for Figure S4.
* Supplementary ILP Intensity Data and Statistics.xlsx: raw data for Figure S15-S21.
* Figure1.eps: Figure 1
* Figure
2.eps: Figure 2
* Figure3.ai: Figure 3
* Figure
4.eps: Figure 4
* Figure5.eps: Figure 5
* Figure
6.ai: Figure 6
* Figure6B-uncropped.png: Figure 6B uncropped gel
* Figure
S1.eps: Figure S1
* FigureS2.eps: Figure S2
* Figure
S3.eps: Figure S3
* FigureS4.eps: Figure S4
* Figure
S5.eps: Figure S5
* FigureS6.eps: Figure S6
* Figure
S7.eps: Figure S7
* FigureS8.eps: Figure S8
* Figure
S9.eps: Figure S9
* FigureS10.eps: Figure S10
* Figure
S11-ucropped-labeled.png: Figure S11
* FigureS12L-ucropped-labeled.png, FigureS12M-ucropped-labeled.png and FigureS12R-ucropped-labeled.png: Figure S12
* Figure
S13L-ucropped-labeled.png, FigureS13M-ucropped-labeled.png and FigureS13R-ucropped-labeled.png: Figure S13
* FigureS14L-ucropped-labeled.png and FigureS14M-ucropped-labeled.png: Figure S14
* FigureS15.eps: Figure S15
* Figure
S16.eps: Figure S16
* FigureS17.eps: Figure S17
* Figure
S18.eps: Figure S18
* FigureS19.eps: Figure S19
* Figure
S20.eps: Figure S20
* Figure_S21.eps: Figure S21

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