Mountain Lion mtDNA genome USA MAFFT alignment file Public

This alignment file contains five novel mitochondrial DNA (mtDNA) genomes of extinct Pennsylvania mountain lions (Puma concolor), two complete mtDNA genomes from Texas and Florida mountain lions available on Genbank (accession numbers NC_016470 and KP202261 respectively), and 14 mtDNA sequences on Genbank produced by Culver et al., who only sequenced the 16s rRNA, ATPase8, and NADH dehydrogenase subunit 5 gene regions. Sequences were aligned using MAFFT version 7 default settings with the E-INS-i iterative refinement method, which is best for a small number of sequences with multiple conserved domains and large gaps, and verified visually. References: Culver, M., Johnson, W. E., Pecon-Slattery, J. & O’Brien, S. J. Genomic ancestry of the American puma (Puma concolor). J. Hered. 91, 186–97 (2000).

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