Parkinson_2016_CoralNurseryProject Public

This project studied the effects of thermal stress on gene expression in the Staghorn coral (Acropora cervicornis). At four seasonal time points, tissue from at least ten colonies at three nurseries were exposed to three temperature treatments for one hour then preserved in RNALater. RNA was extracted with TRI Reagent then cleaned with a Qiagen RNEasy kit. A subset of samples were chosen and pooled for RNA-Seq. The subset included genotypes from 2 of the 3 nurseries (Upper Keys and Lower Keys), 2 of the 4 seasonal collections (Summer 1 and Winter 1), and all 3 temperature treatments (ambient, hot, and cold), resulting in 12 libraries. For each library, RNA from 7 of the 10+ genotypes at each nursery were pooled (84 samples total). Following the manufacturer’s protocol, 150 bp single reads were generated from oligo(dT)-selected total RNA using the Illumina TruSeq Stranded mRNA HT Kit. mRNA sequencing libraries were individually barcoded, multiplexed in equal quantities, and run on three lanes of the Illumina HiSeq 2000 platform. The data were demultiplexed into 12 individual library files. Keywords: Staghorn coral; Acropora cervicornis; functional genomics; heat stress; RNA sequencing DOI:  https://doi.org/10.5072/FK2SF2ST64

Descriptions

Items in this Collection

Sort the listing of items    
View results as:
List of items in this collection
  Title Date Uploaded Visibility
  2018-02-02 Public
Creator:
Iliana Brigitta Baums
Depositor:
John E Parkinson
Edit Access:
Users: jep295, rko5039, jep295
  2017-10-08 Public
Creator:
Parkinson, John E
Depositor:
John E Parkinson
Edit Access:
Users: jep295
  2017-02-17 Public
Creator:
Parkinson, John and Baums, Illiana
Depositor:
Robert K Olendorf
Edit Access:
Users: ibb3, ibb3, rko5039, rko5039
  2017-02-17 Public
Creator:
Parkinson, John and Baums, Illiana
Depositor:
Robert K Olendorf
Edit Access:
Users: ibb3, ibb3, rko5039, rko5039
  2017-02-16 Public
Creator:
Parkinson, John and Baums, Illiana
Depositor:
Robert K Olendorf
Edit Access:
Users: rko5039, rko5039, ibb3
  2017-02-16 Public
Creator:
Parkinson, John and Baums, Illiana
Depositor:
Robert K Olendorf
Edit Access:
Users: rko5039, rko5039, ibb3
  2017-02-16 Public
Creator:
Parkinson, John and Baums, Illiana
Depositor:
Robert K Olendorf
Edit Access:
Users: ibb3, rko5039, rko5039
  2017-02-16 Public
Creator:
Parkinson, John and Baums, Illiana
Depositor:
Robert K Olendorf
Edit Access:
Users: rko5039, ibb3, rko5039
  2017-02-15 Public
Creator:
Parkinson, John and Baums, Illiana
Depositor:
Robert K Olendorf
Edit Access:
Users: rko5039, ibb3, rko5039
  2017-02-15 Public
Creator:
Parkinson, John and Baums, Illiana
Depositor:
Robert K Olendorf
Edit Access:
Users: rko5039, rko5039, ibb3